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Electrochemical sensor for monitoring the photodegradation of catechol based on DNA-modified graphene oxide

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Abstract

We have immobilized DNA on a glassy carbon electrode (GCE) modified with graphene oxide (GO) to develop an electrochemical biosensor for catechol. Compared to carbon nanotubes, the use of GO dramatically improved the electrooxidative current of the guanine and adenine moieties in DNA but retained the low background current of unmodified GCEs. Factors such as DNA adsorption time, DNA concentration and pH of solution were investigated to optimize experimental conditions. In the presence of catechol, the voltammetric response to DNA was inhibited due to the interaction between DNA and catechol. The response to adenine is linearly proportional to the concentration of catechol in the range from 1.0 × 10−6 to 1.0 × 10−4 mol·L−1. If catechol is degraded by the combined action of UV light and hydrogen peroxide, the response to DNA is restored. Thus, the modified electrode can act as an efficient biosensor for monitoring the degradation of catechol.

GO dramatically improved the electrooxidative current of the guanine and adenine moieties in DNA but retained the low background current of unmodified GCEs. While the DNA/GO-modified electrode was applied to monitor catechol, it showed sensitive response to catechol before and after photodegradation treatment.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 20977037) and the Opening Foundation of State Key Laboratory of Chemo/Biosensing and Chemometrics (Grant No. 200908). The authors thank the Analytical and Testing Center of Huazhong University of Science and Technology for the use of SEM and TEM equipments.

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Luoyu Road 1037, Wuhan, 430074, People’s Republic of China

    Lili Wei, Joanna Borowiec, Lihua Zhu & Jingdong Zhang

  2. State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, 410082, People’s Republic of China

    Jingdong Zhang

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  1. Lili Wei
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  2. Joanna Borowiec
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  3. Lihua Zhu
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  4. Jingdong Zhang
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Correspondence to Jingdong Zhang.

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Wei, L., Borowiec, J., Zhu, L. et al. Electrochemical sensor for monitoring the photodegradation of catechol based on DNA-modified graphene oxide. Microchim Acta 173, 439–443 (2011). https://doi.org/10.1007/s00604-011-0580-9

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  • DOI: https://doi.org/10.1007/s00604-011-0580-9

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