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Highly sensitive deoxynivalenol immunosensor based on a glassy carbon electrode modified with a fullerene/ferrocene/ionic liquid composite

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Abstract

We describe a sensitive electrochemical immunosensor for the detection of deoxynivalenol (DON). It is based on a glassy carbon electrode modified with a composite made from fullerene (C60), ferrocene and the ionic liquid. The components were immobilized on the surface of the electrode using chitosan cross-linked with epichlorohydrin. Then, the antibody to DON was covalently conjugated to the surface which then was blocked with serum albumin. The performance of the immunosensor was investigated by cyclic voltammetry and electrochemical impedance spectroscopy. It offers good repeatability (RSD = 1.2%), selectivity, a stability of more than 180 days, an impedimetric response to DON in the range of 1 pgmL−1 to 0.3 ng mL−1, and a detection limit (at S/N = 3) of 0.3 pgmL−1. The limit of detection is better than that of GC, HPLC, GC-MS, HPLC-MS and LC-MS-MS. The effects of omitting C60 or the ionic liquid were also examined. The results indicate that the sensitivity of the biosensor is 2-fold better if C60 and ionic liquids are used. This demonstrates that C60 facilitates electron transfer on the surface of the modified electrode due to its unique electrochemical properties, while the ionic liquid provides a biocompatible microenvironment for the antibody. This results in increased sensitivity and stability. The method was satisfactorily applied to the determination of DON in food samples.

Fullerene, ferrocene, chitosan and ionic liquid offer remarkable synergistic contributions towards improve electrochemical performance of DON sensor. This results that novel sensor exhibits a good repeatability (RSD=1.2%), selectivity, very low detection limit (S/N=3) of 0.0003 ng mL-1, an impedimetric response to DON in the range from 0.001 ng mL-1 to 0.3 ng mL-1 and a stability of more than 180 days. Cyclic voltammograms of, Ab/C60-FC-IL-GCE a and Ab/FC-IL-GCE b

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Acknowledgement

The authors acknowledge the financial support received from the National Natural Science Foundation of China (No.20771045 and 20676052), the National High Technique Development Plan of 863 (2007AA10Z428), sponsored by Qing Lan Project and the Natural Science Foundation of Zhejiang Province (No.Y4080404).

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Authors and Affiliations

  1. School of Chemical and Materials Engineering, Jiangnan University, Wuxi, 214122, China

    Wei Zhilei, Li Zaijun & Liu Junkang

  2. State Key Laboratory of Food Science and Technology, Wuxi, 214122, China

    Sun Xiulan & Li Zaijun

  3. Zhejiang Zanyu Technology Limited Corporation, Hangzhou, 311215, China

    Fang Yinjun, Ren Guoxiao & Huang Yaru

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  1. Wei Zhilei
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  2. Sun Xiulan
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  3. Li Zaijun
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  4. Fang Yinjun
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  5. Ren Guoxiao
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  6. Huang Yaru
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  7. Liu Junkang
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Correspondence to Li Zaijun.

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Zhilei, W., Xiulan, S., Zaijun, L. et al. Highly sensitive deoxynivalenol immunosensor based on a glassy carbon electrode modified with a fullerene/ferrocene/ionic liquid composite. Microchim Acta 172, 365–371 (2011). https://doi.org/10.1007/s00604-010-0495-x

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  • DOI: https://doi.org/10.1007/s00604-010-0495-x

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