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A novel immunosensor for carcinoembryonic antigen based on poly(diallyldimethylammonium chloride) protected prussian blue nanoparticles and double-layer nanometer-sized gold particles

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Abstract

A highly sensitive amperometric immunosensor has been developed for the detection of carcinoembryonic antigen (CEA). It is based on (a) Prussian Blue nanoparticles coated with poly(diallyldimethylammonium chloride) (P-PB) and (b) double-layer gold nanocrystals. The sensor was obtained by first electrodepositing porous gold nanocrystals on the glassy carbon electrode (GCE), and then by modifying the electrode with the coated P-PB. Subsequently, colloidal gold nanoparticles (nano-Au) were adsorbed onto the GCE by electrostatic interactions between the negatively charged nano-Au and the positively charged P-PB to immobilize CEA antibodies. Finally, bovine serum albumin was employed to block possible remaining active sites and to prevent the non-specific adsorption on the nano-Au. This immunosensor was characterized by cyclic voltammetry and scanning electron microscopy. The working range was adjusted to two concentration ranges, viz. from 0.5 to 10 ng.mL−1, and from 10 to 120 ng.mL−1 of CEA, with a detection limit of 0.2 ng.mL−1 at three times the background noise.

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Acknowledgement

This work was supported by the National Natural Science Foundation of China (20675064), the Ministry of Education of China (project 708073), the Natural Science Foundation of Chongqing City (CSTC-2009BA1003). China and High Technology project Foundation of Southwest University (XSGX 02).

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Authors and Affiliations

  1. The Key Laboratory of Luminescence and Real-Time Analysis of MOE, College of Chemical Engineering, Southwest University, Chongqing, 400715, China

    Ping Lv, Ligen Min, Ruo Yuan, Yaqin Chai & Shihong Chen

  2. The Key Laboratory of Eco-environments in Three Gorges Reservoir Region, College of Chemical Engineering, Southwest China University, Chongqing, 400715, China

    Ping Lv, Ligen Min, Ruo Yuan, Yaqin Chai & Shihong Chen

Authors
  1. Ping Lv
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  2. Ligen Min
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  3. Ruo Yuan
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  4. Yaqin Chai
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  5. Shihong Chen
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Correspondence to Ruo Yuan.

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Lv, P., Min, L., Yuan, R. et al. A novel immunosensor for carcinoembryonic antigen based on poly(diallyldimethylammonium chloride) protected prussian blue nanoparticles and double-layer nanometer-sized gold particles. Microchim Acta 171, 297–304 (2010). https://doi.org/10.1007/s00604-010-0435-9

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  • DOI: https://doi.org/10.1007/s00604-010-0435-9

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