Skip to main content
SpringerLink
Log in

Glucose biosensor based on graphite electrodes modified with glucose oxidase and colloidal gold nanoparticles

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

The electrochemistry of glucose oxidase (GOx) immobilized on a graphite rod electrode modified by gold nanoparticles (Au-NPs) was studied. Two types of amperometric glucose sensors based on GOx immobilized and Au-NPs modified working electrode (Au-NPs/GOx/graphite and GOx/Au-NPs/graphite) were designed and tested in the presence and the absence of N-methylphenazonium methyl sulphate in different buffers. Results were compared to those obtained with similar electrodes not containing Au-NPs (GOx/graphite). This study shows that the application of Au-NPs increases the rate of mediated electron transfer. Major analytical characteristics of the amperometric biosensor based on GOx and 13 nm diameter Au-NPs were determined. The analytical signal was linearly related to glucose concentration in the range from 0.1 to 10 mmol L−1. The detection limit for glucose was found within 0.1 mmol L−1 and 0.08 mmol L−1 and the relative standard deviation in the range of 0.1–100 mol L−1 was 0.04–0.39%. The τ1/2 of V max characterizes the storage stability of sensors: this parameter for the developed GOx/graphite electrode was 49.3 days and for GOx/Au-NPs/graphite electrode was 19.5 days. The sensor might be suitable for determination of glucose in beverages and/or in food.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Wang F, Hu S (2009) Electrochemical sensors based on metal and semiconductor nanoparticles. Microchim Acta 165:1

    Article  CAS  Google Scholar 

  2. Wang J (2005) Nanomaterial-based electrochemical biosensors. Analyst 130:421

    Article  CAS  Google Scholar 

  3. Pumera M, Sánchez S, Ichinose I, Tang J (2007) Electrochemical nanobiosensors. Sens Actuat B 123:1195

    Article  Google Scholar 

  4. Wang Y, Xu H, Zhang J, Li G (2008) Electrochemical sensors for clinic analysis. Sensors 8:2043

    Article  CAS  Google Scholar 

  5. Bakker E, Qin Y (2006) Electrochemical sensors. Anal Chem 78:3965

    Article  CAS  Google Scholar 

  6. Luo X, Morrin A, Killard AJ, Smyth MR (2006) Application of nanoparticles in electrochemical sensors and biosensors. Electroanalysis 18:319

    Article  CAS  Google Scholar 

  7. Kurniawan F, Tsakova V, Mirsky VM (2006) Gold nanoparticles in nonenzymatic electrochemical detection of sugars. Electroanalysis 18:1937

    Article  CAS  Google Scholar 

  8. Freire RS, Pessoa CA, Mello LD, Kubota LT (2003) Direct electron transfer: an approach for electrochemical biosensors with higher selectivity and sensitivity. J Braz Chem Soc 14:230

    Article  CAS  Google Scholar 

  9. Onda M, Lvov Y, Ariga K, Kunitake T (1996) Sequential actions of glucose oxidase and peroxidase in molecular films assembled by layer-by-layer alternate adsorption. Biotechnol Bioeng 51:163

    Article  Google Scholar 

  10. Ramanavicius A (2007) Amperometric biosensor for the determination of creatine. Anal Bioanal Chem 387:1899

    Article  CAS  Google Scholar 

  11. Hoshi T, Sagae N, Daikuhara K, Takahara K, Anzai J (2007) Multilayer membranes via layer-by-layer deposition of glucose oxidase and Au nanoparticles on a Pt electrode for glucose sensing. Mater Sci Eng C 27:890

    Article  CAS  Google Scholar 

  12. Ramanaviciene A, Schuhmann W, Ramanavicius A (2006) AFM study of conducting polymer polypyrrole nanoparticles formed by redox enzyme – glucose oxidase – initiated polymerisation. Coll Surf B 48:159

    Article  CAS  Google Scholar 

  13. Liu S, Ju H (2003) Reagentless glucose biosensor based on direct electron transfer of glucose oxidase immobilized on colloidal gold modified carbon paste electrode. Biosens Bioelectron 19:177

    Article  CAS  Google Scholar 

  14. Zhao S, Zhang K, Bai Y, Yang W, Sun C (2006) Glucose oxidase/colloidal gold nanoparticles immobilized in Nafion film on glassy carbon electrode: direct electron transfer and electrocatalysis. Bioelectrochemistry 69:158

    Article  CAS  Google Scholar 

  15. Zhao J, O’Daly JP, Henkens RW, Stonehuerner J, Crumbliss AL (1996) A xanthine oxidase/colloidal gold enzyme electrode for amperometric biosensor applications. Biosens Bioelectron 11:493

    Article  CAS  Google Scholar 

  16. Yáñez-Sedeño P, Pingarrón JM (2005) Gold nanoparticle-based electrochemical biosensors. Anal Bioanal Chem 382:884

    Article  Google Scholar 

  17. Zhang S, Wang N, Yu H, Niu Y, Sun C (2005) Covalent attachment of glucose oxidase to an Au electrode modified with gold nanoparticles for use as glucose biosensor. Bioelectrochemistry 67:15

    Article  CAS  Google Scholar 

  18. Zhang S, Wang N, Niu Y, Sun C (2005) Immobilization of glucose oxidase on gold nanoparticles modified Au electrode for the construction of biosensor. Sens Actuat B 109:367

    Article  Google Scholar 

  19. Guo S, Wang E (2007) Synthesis and electrochemical applications of gold nanoparticles. Anal Chim Acta 598:181

    Article  CAS  Google Scholar 

  20. Mena ML, Yáñez-Sedeño P, Pingarrón JM (2005) A comparision of different strategies for the construction of amperometric enzyme biosensors using gold nanoparticle-modified electrodes. Anal Biochem 336:20

    Article  CAS  Google Scholar 

  21. Ma SY, Lu WS, Mu J, Liu F, Jiang L (2008) Inhibition and enhancement of glucose oxidase activity in a chitosan-based electrode filled with silver nanoparticles. Coll Surf A-Physicochem Eng Asp 324:9

    Article  CAS  Google Scholar 

  22. Tsai YC, Chen SY, Lee CA (2008) Amperometric cholesterol biosensors based on carbon nanotube-chitosan-platinum-cholesterol oxidase nanobiocomposite. Sens Actuat B 135:96

    Article  Google Scholar 

  23. Bahshi L, Frasconi M, Tel-Vered R, Yehezkeli O, Willner I (2008) Following the biocatalytic activity of glucose oxidase by electrochemically cross-linked enzyme-Pt nanoparticles composite electrodes. Anal Chem 80:8253

    Article  CAS  Google Scholar 

  24. Vertegel AA, Siegel RW, Dordick JS (2004) Silica nanoparticle size influences the structure and enzymatic activity of adsorbed lysozyme. Langmuir 20:6800

    Article  CAS  Google Scholar 

  25. Willner I, Willner B, Katz E (2007) Biomolecule-nanoparticle hybrid systems for bioelectronic applications. Bioelectrochemistry 70:2

    Article  CAS  Google Scholar 

  26. Xiao Y, Patolsky F, Katz E, Hainfeld JF, Willner I (2003) “Plugging into enzymes”: nanowiring of redox enzymes by a gold nanoparticle. Science 299:1877

    Article  CAS  Google Scholar 

  27. Thibault S, Aubriet H, Arnoult C, Ruch D (2008) Gold nanoparticles and a glucose oxidase based biosensor: an attempt to follow-up aging by XPS. Microchim Acta 163:211

    Article  CAS  Google Scholar 

  28. Wu S, Zhong Z, Wang D, Li M, Qing Y, Dai N, Li Z (2009) Gold nanoparticle-labeled detection antibodies for use in an enhanced electrochemical immunoassay of hepatitis B surface antigen in human serum. Microchim Acta 166:269

    Article  CAS  Google Scholar 

  29. Wang J (2003) Nanoparticle-based electrochemical DNA detection. Anal Chim Acta 500:247

    Article  CAS  Google Scholar 

  30. Liu S, Leech D, Ju H (2003) Application of colloidal gold in protein immobilization, electron transfer, and biosensing. Anal Lett 36:1

    Article  CAS  Google Scholar 

  31. Wu S, Zhong Z, Wang D, Li M, Qing Y, Dai N, Li Z (2009) Gold nanoparticle-labeled detection antibodies for use in an enhanced electrochemical immunoassay of hepatitis B surface antigen in human serum. Microchim Acta 166:269

    Article  CAS  Google Scholar 

  32. Zhang F, Cho SS, Yang SH, Seo SS, Cha GS, Nam H (2006) Gold nanoparticle-based mediatorless biosensor prepared on microporous electrode. Electroanalysis 18:217

    Article  Google Scholar 

  33. Xiao Y, Ju H-X, Chen H-Y (1999) Hydrogen peroxide sensor based on horseradish peroxidase-labeled Au colloids immobilized on gold electrode surface by cysteamine monolayer. Anal Chim Acta 391:73

    Article  CAS  Google Scholar 

  34. Ramanaviciene A, Nastajute G, Snitka V, Kausaite A, German N, Barauskas-Memenas D, Ramanavicius A (2009) Spectrophotometric evaluation of gold nanoparticles as red-ox mediator for glucose oxidase. Sens Actuat B 137:483

    Article  Google Scholar 

  35. Yang W, Wang J, Zhao S, Sun Y, Sun C (2006) Multilayered construction of glucose oxidase and gold nanoparticles on Au electrodes based on layer-by-layer covalent attachment. Electrochem Commun 8:665

    Article  CAS  Google Scholar 

  36. Ramanavicius A, Kausaite A, Ramanaviciene A (2008) Self-encapsulation of oxidases as a basic approach to tune the upper detection limit of amperometric biosensors. Analyst 133:1083

    Article  CAS  Google Scholar 

  37. Neiman B, Grushka E, Lev O (2001) Use of gold nanoparticles to enhance capillary electrophoresis. Anal Chem 73:5220

    Article  CAS  Google Scholar 

  38. Zhou Y-G, Yang S, Qian Q-Y, Xia X-H (2009) Gold nanoparticles integrated in a nanotube array for electrochemical detection of glucose. Electrochem Commun 11:216

    Article  CAS  Google Scholar 

  39. Krikstopaitis K, Kulys J, Tetianec L (2004) Bioelectrocatalytical glucose oxidation with phenoxazine modified glucose oxidase. Electrochem Commun 6:331

    Article  CAS  Google Scholar 

  40. Ramanavicius A, Ramanaviciene A (2009) Hemoproteins in design of biofuel cells. Fuel Cells 9:25

    Article  CAS  Google Scholar 

Download references

Acknowledgement

This work was financially supported by Lithuanian State Science and Studies Foundation project number S-6/2007 and Agency for International Science and Technology Development Programmes in Lithuania for performance of COST program D43.

Author information

Authors and Affiliations

  1. Faculty of Chemistry, Center of Nanotechnology and Materials Science—NanoTechnas, Vilnius University, Naugarduko 24, LT-03225, Vilnius, Lithuania

    Natalija German, Almira Ramanaviciene, Jaroslav Voronovic & Arunas Ramanavicius

  2. Laboratory of Immunoanalysis and Nanotechnology, Institute of Immunology of Vilnius University, Moletu road 29, LT-08409, Vilnius, Lithuania

    Natalija German

Authors
  1. Natalija German
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Almira Ramanaviciene
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jaroslav Voronovic
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Arunas Ramanavicius
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Natalija German or Arunas Ramanavicius.

Rights and permissions

Reprints and permissions

About this article

Cite this article

German, N., Ramanaviciene, A., Voronovic, J. et al. Glucose biosensor based on graphite electrodes modified with glucose oxidase and colloidal gold nanoparticles. Microchim Acta 168, 221–229 (2010). https://doi.org/10.1007/s00604-009-0270-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00604-009-0270-z

Keywords

Search

Navigation