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Fucosylation is associated with the malignant transformation of intraductal papillary mucinous neoplasms: a lectin microarray-based study

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Abstract

Purpose

Intraductal papillary mucinous neoplasm (IPMN) is an intraductal mucin-producing pancreatic neoplasm with the potential for malignant transformation. Changes in glycans expressed on the cell surface and glycotransferases play important roles in malignant transformation. We conducted this study to analyze glycan alterations in IPMNs by using a lectin microarray and to identify the factors associated with altered glycans and their relationships with malignant transformation.

Methods

Using a lectin microarray, we evaluated glycan expression in 22 samples of IPMN with carcinoma, obtained from curative resections performed in our department. We also used immunohistochemistry to investigate fucosyltransferase 8 (Fut 8) protein expression, which is associated with glycan alterations in IPMNs.

Results

The lectin microarray demonstrated that only two lectins, Aleuria aurantia lectin (AAL) and Aspergillus oryzae l-fucose-specific lectin (AOL), which bind to fucose, exhibited significant sequential increases from normal pancreatic duct to adenoma and carcinoma. Similarly, Fut 8 protein expression, which is associated with AAL and AOL, sequentially and significantly increased from the normal pancreatic duct to adenoma and carcinoma.

Conclusions

Lectin microarray analysis suggested that fucosylation is associated with the malignant transformation of IPMNs.

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Acknowledgments

We thank Ms. Mayumi Takeda, Yuiko Aso, and Hiroko Taguchi for their technical assistance.

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Authors and Affiliations

  1. Department of Gastroenterological and Pediatric Surgery, Oita University Faculty of Medicine, Idaigaoka, Hasama-machi, Yufu, Oita, 879-5593, Japan

    Kiminori Watanabe, Masayuki Ohta, Kazuhiro Yada, Yoko Komori, Yukio Iwashita & Masafumi Inomata

  2. Department of Pathology, Oita Prefectural Hospital, Oita, Japan

    Kenji Kashima

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  1. Kiminori Watanabe
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  2. Masayuki Ohta
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  3. Kazuhiro Yada
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Correspondence to Kiminori Watanabe.

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Watanabe, K., Ohta, M., Yada, K. et al. Fucosylation is associated with the malignant transformation of intraductal papillary mucinous neoplasms: a lectin microarray-based study. Surg Today 46, 1217–1223 (2016). https://doi.org/10.1007/s00595-015-1299-8

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  • DOI: https://doi.org/10.1007/s00595-015-1299-8

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