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Trehalose does not affect the functions of human neutrophils in vitro

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Abstract

Purpose

Trehalose, naturally occurring disaccharide, has been reported to prevent postoperative abdominal adhesions in animal models. We investigated whether trehalose affects the function of human polymorphonuclear neutrophils (PMNs) in vitro to assess the feasibility of its clinical application as an anti-adhesive barrier.

Methods

Human PMNs were obtained from 17 healthy volunteers. Escherichia coli and Staphylococcus aureus were used for the bacterial infection model, whereas lipopolysaccharide (LPS) and interleukin (IL)-1β were used for inflammation induction model. The PMN phagocytosis rates of bacteria and apoptosis/necrosis were assessed on trehalose, maltose, and control media. Cytokines; namely, tumor necrosis factor-α, IL-1α, IL-1Ra, IL-6, and IL-8; and PMN-elastase were measured on each medium in both models.

Results

There were no significant differences in the phagocytosis rates, apoptosis/necrosis rates, or levels of all cytokines or PMN-elastase among the three media in the bacterial infection model. There were also no significant differences in the levels of all cytokines and PMN-elastase among the three media in the IL-1β inflammation induction model. PMN-elastase was lower in trehalose and maltose medium after LPS stimulation, at 3 and 24 h.

Conclusions

Our results suggest that trehalose does not affect the cellular function, cytokine production, or release of PMN-elastase of human PMNs in an in vitro bacterial infection model.

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Abbreviations

TNF-α:

Tumor necrosis factor-α

IL-1α:

Interleukin-1α

IL-1Ra:

IL-1 receptor antagonist

LPS:

Lipopolysaccharide

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Acknowledgments

This study was funded by Otsuka Pharmaceutical Factory, Inc. (Tokushima, Japan). Trehalose and maltose were supplied by Otsuka Pharmaceutical Factory, Inc.

Conflict of interest

Masato Kusunoki consults to other clinical trials about the efficacy and safety of trehalose in the prevention of postsurgical adhesion formation conducted by Otsuka Pharmaceutical Factory, Inc. (Tokushima, Japan). Masato Kusunoki received a research grant for this study from Otsuka Pharmaceutical Factory, Inc.

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Authors and Affiliations

  1. Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie, 514-8507, Japan

    Koji Tanaka, Mikio Kawamura, Kohei Otake, Yuji Toiyama, Yoshinaga Okugawa, Yasuhiro Inoue, Keiichi Uchida, Toshimitsu Araki, Yasuhiko Mohri & Masato Kusunoki

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  1. Koji Tanaka
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  2. Mikio Kawamura
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  3. Kohei Otake
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  4. Yuji Toiyama
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  5. Yoshinaga Okugawa
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  6. Yasuhiro Inoue
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  7. Keiichi Uchida
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  8. Toshimitsu Araki
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  9. Yasuhiko Mohri
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  10. Masato Kusunoki
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Corresponding author

Correspondence to Koji Tanaka.

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Tanaka, K., Kawamura, M., Otake, K. et al. Trehalose does not affect the functions of human neutrophils in vitro. Surg Today 44, 332–339 (2014). https://doi.org/10.1007/s00595-013-0625-2

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  • DOI: https://doi.org/10.1007/s00595-013-0625-2

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