Abstract
Fish blood is a pathophysiological indicator of the whole body function and is thus considered as an important tool in diagnosing the structural and functional status of fish. However, the blood parameters vary between species to species and it mainly depends upon the favorable environmental conditions where the species live. The purpose of the current study is to evaluate the hematological and serum biochemical indices of five Schizothorax species: Schizothorax labiatus, S. plagiostomus, S. esocinus, S. curvifrons and S. niger in order to establish the resemblances and variations between these Schizothorax species which are inhabiting in river Jhelum. The hematological profile including hemoglobin (Hb), total red blood cell (RBC) count, hematocrit (Hct), white blood cell (WBC) count, erythrocyte sedimentation rate (ESR), and erythrocyte indices: mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH), and mean corpuscular volume (MCV) was analyzed from each Schizothorax sp. Statistical analysis showed that there were significant differences (p < 0.05) in blood parameters among five Schizothorax spp. The results showed lowest values of hematological parameters in S. niger with respect to other species, while the highest values of hematological parameters were recorded in S. plagiostomus. Significant differences (p < 0.05) in serum biochemical levels of glucose, protein, cholesterol and urea were also noted in Schizothorax spp. The differences found in the hematological profile and serum biochemical composition in these fishes can be attributed to the individual feeding behavior, tolerance and environmentally adjustable capability of the fish. However, further study is required to correlate the present study with some other parameters such as the nutrient status of the river Jhelum where these fishes live.
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Adebayo OT, Fagbenro OA, Ajayi CB, Popoola OM (2007) Normal haematological profile of Parachanna obscura as diagnostic tool in aquaculture. Int J Zool Res 3:193–199
Adham K, Khairalla A, Abu-Shabana M, Abdel-Maguid N, Abdel-Moneim A (1997) Environmental stress in lake Maryut and physiological response of Tilapia zilli. J Environ Sci Health A Environ Sci Eng Toxicol 32A:9–10
Adham KG, Ibrahim HM, Hamed SS, Saleh RA (2002) Blood chemistry of the Nile tilapia, Oreochromis niloticus (Linnaeus, 1757) under the impact of water pollution. Aquat Ecol 36:549–557
Akinrotimi OA, Abu O, Ansa EJ, Edun OM, George OS (2009) Hematological responses of Tilapia guineensis to acute stress. Int J Nat Appl Sci 4:338–343
Anver CE (2004) Blood chemistry (electrolytes, lipoprotein and enzymes) values of black scorpion fish (Scorpaena porcus, 1758) in the Dardnelles. Turk J Biol Sci 4:716–719
APHA (American Public Health Association) (1998) Standard methods for the examination of water and wastewater, 20th edn. APHA, Washington, DC
Bahmani M, Kazemi R, Donskaya P (2001) A comparative study of some haematological features in young reared sturgeons (Acipenser persicus and Huso huso). Fish Physiol Biochem 24:135–140
Ballarin L, Dall’Oro M, Bertotto D, Libertini A, Francescon A, Barbaro A (2004) Haematological parameters in Umbrina cirrosa (Teleostei, Sciaenidae): a comparison between diploid and triploid specimens. Comp Biochem Physiol A Mol Integ Physiol 138:45–51
Bani A, Tabarsa M, Falahatkar B, Banan A (2009) Effects of different photoperiods on growth, stress and haematological parameters in juvenile great sturgeon Huso huso. Aquacult Res 40:1899–1907
Bano Y (1985) Sublethal stress of DDT on biochemical composition of catfish Clarias batrachus. Indian J Environ Health 27:230–236
Barcellos LJG, Kreutz LC, Rodrigues LB, Fioreze I, Quevedo RM, Cericato L, Conrad J, Soso AB, Fagundes M, Lacerda LA, Terra S (2003) Haematological and biochemical characteristics of male jundia, Rhamdia quelen (Quoy and Gaimard Pimelodidae): changes after acute stress. Aquacult Res 34:1465–1469
Barton BA (2000) Salmonid fishes differ in their cortisol and glucose responses to handling and transport stress. N Am J Aquacult 62:218
Barton BA, Iwama GK (1991) Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Annu Rev Fish Diss 1:3–26
Barton BA, Schreck CB, Barton LD (1987) Effects of chronic cortisol administration and daily acute stress on growth, physiological conditions, and stress responses in juvenile rainbow trout. Diss Aquat Org 2:173–185
Bartonkova J, Hyrsl P, Vojtek L (2016) Glucose determination in fish plasma by two different moderate methods. Acta Vet Brno 85:349–353
Bayir A (2005) Seasonal changes in antioxidant enzyme activities, serum lipids, lipoproteins and haematological parameters of siraz fish (Capoeta capoeta umbla) living in Hinis stream (Murat Basin). Degree Diss., Ataturk University, Turkey
Bayir A, Sirkecioglu AN, Polat H, Aras NM (2007) Biochemical profile of blood serum of siraz Capoeta capoeta umbla. Comp Clin Pathol 16:119–126
Bhaskar BR, Rao KS (1990) Use of hematological parameters as diagnostic tools in determining health of milk fish, Chanos chanos (Forskal) in brakish water culture. Aquacult Fish Manage 21:125–129
Borges A, Scotti LV, Siqueira DR, Jurinitz DF, Wassermann GF (2004) Hematologic and serum biochemical values for jundia´ (Rhamdia quelen). Fish Physiol Biochem 30:21–25
Borkovic SS, Pavlovic SZ, Kovacevic TB, Stajn AS, Petrovic VM, Saicic ZS (2008) Antioxidant defense enzyme activities in hepato pancreas, gills and muscle of spiny cheek crayfish (Orconectes limosus) from the river Danube. Comp Biochem Physiol Part C Toxicol Pharmacol 147:122–128
Burtis CA, Ashwood ER (1996) Tietz fundamentals of clinical chemistry. WB Saunders, Philadelphia
Cech JJ, Bartholow SD, Young PS, Hopkins TE (1996) Stripped bass exercise and handling stress in freshwater: physiological responses to recovery environment. Trans Am Fish Soc 125:308–320
Chaudhuri SH, Pandit T, Benerjee S (1986) Size and sex related variations of some blood parameters of Sarotheriodon massambica. Environ Ecol 4:61–63
Chen YE, Jin S, Wang GL (2005) Study on blood physiological and biochemical indices of vibrio alginilyticus disease of Lateolabrax japonicas. J Oceanogr Taiwan Str 24:104–108
Cnaani A, Tinman S, Avidar Y, Ron M, Hulata G (2004) Comparative study of biochemical parameters in response to stress in O.aureus, O.mossambicus and two strains of O.niloticus. Aquacult Res 35:1434–1440
Coles EH (1986) Veterinary clinical pathology. W.B. Saunders, Philadelphia, pp 1–42
Coulombe JJ, Favreau L (1963) A new simple semimicro method for colorimetric determination of urea. Clin Chem 9:8–102
Coz-Rakovac R, Teskeredzic R (2000) Biochemical changes in coho salmon plasma following sea water adaptation. Period Biol 102:297–301
Coz-Rakovac R, Strunjak-perovic I, Hacmanjek M, Topic PN, Lipej Z, Sostaric B (2005) Blood chemistry and histological properties of wild and cultured sea bass (Dicentrarchus labrax) in the north Adriatic Sea. Vet Res Com 29:677–687
Dacie JV, Lewis SM (1975) Basic haematological techniques. In: Practical haematology, 5th edn. ELBS and Churchill Livingstone, pp 21–67
Das BC (1965) Age related trends in the blood chemistry and hematology of the Indian carp (Catla catla). Gerontologia 10:47–64
Das PC, Ayyappan S, Jena JK (2006) Haematological changes in the three Indian major carps, Catla catla (Hamilton), Labeo rohita (Hamilton) and Cirrhinus mrigala (Hamilton) exposed to acidic and alkaline water pH. Aquaculture 256:80–87
Duthie GG, Tort L (1985) Effect of dorsal aortic connotation on the respiration and hematology of the Mediterranean dog-fish Scyliorhinus canicula. Comp Biochem Physiol 81:879–883
Edsall CC (1999) A blood chemistry profile for lake trout. J Aquat Anim Health 11:81–86
Fazio F, Faggio C, Marafioti S, Torre A, Sanfilippo M, Piccione G (2012) Comparative study of haematological profile on Gobius niger in two different habitat sites: Faro Lake and Tyrrhenian Sea. Cah Biol Mar 53:213–219
Filiciotto F, Fazio F, Marafioti S, Buscaino G, Maccarrone V, Faggio C (2012) Assessment of hematological parameter range values using an automatic method in European sea bass (Dicentrarcbus labrax L.). Natura Rerum 1:29–36
Francesco F, Satheeshkumar P, Kumar DS, Caterina F, Giuseppe P (2012) A comparative study of hematological and blood chemistry of Indian and Italian Grey Mullet (Mugil cephalus Linneaus 1758). HOAJ Biol 1:1–5
Gallardo MA, Sala-Rabanal M, Ibarz A, Padrós F, Blasco J, Fernández-Borra J, Sánchez J (2003) Functional alterations associated with “winter syndrome” in gilthead sea bream (Sparus aurata). Aquaculture 223:15–27
Gul Y, Gao ZX, Qian XQ, Wang WM (2011) Haematological and serum biochemical characterization and comparison of wild and cultured northern snakehead (Channa argus Cantor, 1842). J Appl Ichthyol 27:122–128
Hardy D, Audet C (1990) Evaluation of plasma glucose as an indicator of mild chronic stress in brook charr (Salvelinus fontinalis). Bull Aquacult Assoc Can 90:54–56
Henry R, Canon DC, Winkelman JW (1974) The colorimetric determination of serum triglycerides. Clin Chem 29:538–542
Hill S (1982) A literature review of the blood chemistry of rainbow trout, Salmo gairdneri. J Fish Biol 20:535–569
Hrubec TC, Smith SA, Robertson JL (2001) Age related changes in hematology and plasma chemistry values of hybrid striped bass (Morone chrysops · Morone saxatilis). Vet Clin Pathol 30:8–15
Jawad LA, Al-Mukhtar MA, Ahmed HK (2004) The rela-tionship between haematocrit and some biological parameters of the Indian shad,Tenualosa ilisha (FamilyClupeidae). Anim Biodi Conserv 27:47–52
Joseph John P (2007) Alteration of certain blood parameters of freshwater teleost Mystus vittatus after chronic exposure to Metasystox and Sevin. Fish Physiol Biochem 33:15–20
Kareem OK, Ajani EK, Orisasona O, Olanrewaju AN (2015) The sex ratio, gonadosomatic index, diet composition and fecundity of African Pike, Hepsetus odoe (Bloch, 1794) in Eleyele lake, Nigeria. J Fish Livest Prod:1–4
Kavya KS, Kulkarni RS, Jadesh M (2015) Some blood biochemical changes in response to saline exposure in the fresh water fish, Notopterus notopterus (Pallas). Int Let Nat Sci 49p
Langston AL, Hoare R, Stefansson M, Fitzgerald R, Wergeland H, Mulcahy M (2002) The effect of temperature on non-specific defence parameters of three strains of juvenile Atlantic halibut (Hippoglossus hippoglossus L.). Fish Shellfish Immunol 12:61–76
Lavanya S, Ramesh M, Kavitha C, Malarvizhi A (2011) Hematological, biochemical and ion regulatory responses of Indian major carp, Catla catla during chronic sublethal exposure to inorganic arsenic. Chemosphere 82:977–985
Lermen CL, Lappe R, Crestani M, Vieira VP, Gioda CR, Schetinger MRC, Baldisserotto B, Moraes G, Morsch VM (2004) Effect of different temperature regimes on metabolic and blood parameters of silver catfish Rhamdia quelen. Aquaculture 239:497–507
Magill AH, Sayer MDJ (2004) The effect of reduced temperature and salinity on the blood physiology of juvenile Atlantic cod. J Fish Biol 64:1193–1205
Masopust J (2000) Clinical biochemistry ( in Czech ) Karolinium. Prague:832 pp
Natt MP, Herrick CA (1952) A new blood diluent for counting erythrocyte and leucocytes of chicken. Poultry Sci 31:735–738
Ojolick EJ, Cusack R, Benfey TJ, Kerr SR (1995) Survival and growth of all female diploid and triploid Clarias macrocephalus. Fish Genet Biotropical Spec Publica 52:79–86
Orun I, Dorucu M, Yazlak H (2003) Hematological parameters of three cyprinid fish species from Karakaya dam lake, Turkey. J Biol Sci 3:320–328
Pal A, Parida SP, Swain MM (2008) Hematological and plasma biochemistry in fan-throated lizard Sitana ponticeriana (Sauria:Agamidae). Russ J Herpetol 2:110–116
Parida SP, Dutta SK, Pal A (2011) Hematological and plasma biochemistry and plasma biochemistry in Psammophilus blanfordanus (Sauria:Agamidae). Compe Clin Pathol 21:1387–1394. https://doi.org/10.1007/s00580-011-1303-7
Peres H, Santos S, Oliva-Teles A (2014) Blood chemistry profile as indicator of nutritional status in European seabass (Dicentrarchus labrax). Fish Physiol Biochem 40:1339–1347
Porchas M, Cordova L, Enriquez R (2009) Cortisol and glucose: reliable indicators of fish stress. Pan-Amer J Aquat Sci 4:158–178
Pradhan SC, Patra AK, Sarkar B, Pal A (2012) Seasonal changes in hematological parameters of Catla catla (Hamilton 1822). Comp Clin Pathol A 21:1473–1487
Pradhan SC, Patra AK, Mohanty KC, Pal A (2014a) Hematological and plasma biochemistry in Cirrhinus mrigala (Hamilton 1822). Comp Clin Pathol 23:509–518
Pradhan SC, Patra AK, Pal A (2014b) Hematological and plasma chemistry of Indian major carp, Labeo rohita (Hamilton, 1822). J Appl Ichthyol 30:48–54
Prasad G, Charles S (2010) Haematology and leucocyte enzyme cytochemistry of a threatened yellow catfish Horabagrus brachysoma (Gunther 1864). Fish Physiol Biochem 36:435–443
Raizada MN, Jain KK, Raizada S (1983) Monthly variations in the hematocrit values (PCV) in a teleost, Cirrhinus mrigala (Ham.). J Comp Physiol 3:196–198
Rajaguru A (1992) Biology of two co-occurring tongue fishes, Cynoglossus arel and C. lida (Pleuronectiformes: Cynoglossidae), from Porto Nova, southeast coast of India. Fish Bull 90:328–367
Rambhaskar B, Srinivasa Rao K (1986) Comparative haematology of ten species of marine fish from Visakhapatnam Coast. J Fish Biol 30:59–66
Rehulka J, Minarik B (2005) Blood parameters in brook trout Salvelinus fontinalis (Mitchill, 1815), affected by columnaris disease. Aquacult Res 38:1182–1197
Richter H, Luckstadt C, Focken UL, Becker K (2000) An improved procedure to assess fish condition on the basis of length-weight relationships. Arch Fish Mar Res 48:226–235
Robertson OH, Krupp NA, Favour CB, Hane S, Thomas SF (1961) Physiological changes occurring in the blood of the pacific salmon, Oncorhynchus tshawytscha accompanying sexual maturation and spawning. Endocrinol 68:325–337
Santos MA, Pacheco M (1996) Anguilla anguilla L. stress biomarkers recovery in clean water and secondary treated pulp mill effluent. Ecotoxicol Environ Saf 35:96–100
Satheeshkumar P, Ananthan G, Senthil Kumar D, Jagadeesan L (2012) Hematology and biochemical parameters of different feeding behavior of teleost fishes from Vellar estuary, India. Comp Clin Pathol 21:1187–1191
Shah SL, Altindag A (2005) Alteration of immunological parameters of tench (Tinca tinca) after acute and chronical exposure to lethal and sublethal treatments with mercury, cadmium and lead. Turk J Vet Anim Sci 29:1163–1168
Sharma NK, Akhtar MS, Pandey NN, Singh R, Singh AK (2017) Sex specific seasonal variation in hematological and serum biochemical indices of Barilius bendelisis from Central Himalaya, India. Proc Natl Acad Sci India Sect B Biol Sci 87:1185–1197
Silverira-Coffigny R, Prieto-Trujillo A, Ascencio-Valle F (2004) Effects of different stressors in haematological variables in cultures Oreochromis aureus S. Comp Biochem Physiol C 139:245–250
Stillwell EJ, Benfey TJ (1995) Hemoglobin level, metabolic rate and swimming performance in triploid brook trout (Salvelinus fontinalis). Aquaculture 137:358–358
Svetina A, Matasin Z, Tofant A, Vucemilo M, Fijan N (2002) Haematology and some blood chemical parameters of young carp till the age of three years. Acta Vet Hung 50:459–467. https://doi.org/10.1556/AVet.50.2002.4.8
Svobodova Z (1977) Influence of sex on the glucosemia and glycogen content in hepatopancreas and musculature of the carp Cyprinus carpio L. Acta Vet Brno 46:253–258
Svobodova Z, Flajshans M, Kolarova J, Modra H, Svoboda M, Vajcova V (2001) Leukocyte profile of diploid and triploid tench, Tinca tinca L. Aquaculture 198:159–168
Svobodova Z, Vykusova B, Modra H, Jarkovsky J, Smutna M (2006) Haematological and biochemical profile of harvest- size carp during harvest and post-harvest storage. Aquacult Res 37:959–965
Svobodova Z, Kroupova H, Modra H, Flajshans M, Randak T, Savina LV, Gela D (2008) Haematological profile of common carp spawners of various breeds. J Appl Ichthyol 24:55–59
Torres P, Tort L, DePauw N, Joyce J (1991) Effects of stress and metal exposure on blood parameters and liver metabolism in rainbow trout. Spec Publ Europ Aquacult Soc 14:312–313
Triander P (1969) Determination of glucose in blood using glucose oxidase with an alternate oxygen receptor. Ann Clin Biochem 6:24–27
Tripathi NK (2003) Pathogenesis and treatment of Flavobacterium columnare-induced dermatitis in koi (Doctoral dissertation, uga)
Vazquez GR, Guerrero GA (2007) Characterization of blood cells and haematological parameters in Cichlasoma dimerus (Teleostei, Perciformes). Tissue Cell 39:151–160
Velisek J, Svobodova Z, Piackova V (2009) Effects of acute exposure to bifenthrin on some haematological, biochemical and histopathological parameters of rainbow trout (Oncorhynchus mykiss). Vet Med Sci 54:131–137
Wagner T, Congleton JL (2004) Blood chemistry correlates of nutritional condition, tissue damage, and stress in migrating juvenile chinook salmon (Oncorhynchus tshawytscha). Candian J Fish Aquac Sci 61:1066–1107
Wedemeyer GA, Gould RW, Yasutake WT (1983) Some potentials and limits of the leucocrit test as a fish health assessment method. J Fish Biol 23:711–716
Wendelaar Bonga SE (1997) The stress response in fish. Physiol Rev 77:591–625
Witeska M (2013) Erythrocytes in teleost fishes: a review. Zool Ecol 23:275–281
Xiaoyun Z, Mingyun L, Khalid A, Weinmin W (2009) Comparison of haematology and serum biochemistry of cultured and wild dojo loach Misgurnus anguillicaudatus. Fish Physiol Biochem 35:435–444
Yakhnenko VM, Yakhnenko MS (2006) Haematological parameters of lake Baikal oil fish (Golomyanka) (Comephorus dybowskii and Comephorus baicalensis). Hydrobiologia 568:233–237
Yang XP (2002) Animal physiology. Higher Education, Beijing (in Chinese)
Yin J, Zhao ZS, Chen XQ, Li YQ, Zhu LY (2005) Karyotype comparison of diploid and tetraploid loach, Misgurnus anguillicanudatus. Acta Hydrobiol Sin 29:469–472
Yousefzadeh F, Khara H (2014) Changes in blood chemistry and hematological indices of Capoeta capoeta gracilis in relation to age, sex, and geographic location. Comp Clin Pathol 24:1–5
Zakes Z, Demska-Zakes K, Szczepkowski M, Rożynski M, Ziomek E (2016) Impact of sex and diet on hematological and blood plasma biochemical profiles and liver histology of pikeperch (Sander lucioperca (L.)). Arch Pol Fish 24:61–68
Zarejabad AM, Sudagar M, Pouralimotlagh S, Bastami KD (2010) Effects of rearing temperature on hematological and biochemical parameters of great sturgeon (Huso huso Linnaeus,1758) juvenile. Comp Clin Pathol 19:367–371
Zuim SMF, Rosa AAM, Castagnolli N (1988) Sex and sexual cycle influences over metabolic parameters in pacu Piaractus mesopotamicus (Holmberg, 1887). Proc Aquacult Int Cong Vancou:74
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We are thankful to the Head, Department of Zoology, University of Kashmir, for providing necessary laboratory facilities.
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The authors were financially supported by the UGC, New Delhi, in the form of “Himalayan Biodiversity—Documentation, Bio-prospection & Conservation” under the scheme Centre with Potential for Excellence in Particular Areas (CPEPA).
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Ahmed, I., Sheikh, Z.A. Hematological and serum biochemical parameters of five freshwater snow trout fish species from river Jhelum of Kashmir Himalaya, India. Comp Clin Pathol 28, 771–782 (2019). https://doi.org/10.1007/s00580-019-02909-y
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DOI: https://doi.org/10.1007/s00580-019-02909-y