Skip to main content
SpringerLink
Log in

Hematological and plasma biochemistry in Psammophilus blanfordanus (Sauria: Agamidae)

  • Original Article
  • Published:
Comparative Clinical Pathology Aims and scope Submit manuscript

Abstract

The purpose of this study is to determine reference ranges for hematological and plasma chemistry of Psammophilus blanfordanus (Sauria: Agamidae) collected from their natural habitat. Blood samples were taken from coccygeal vein and lower abdominal vein of P. blanfordanus and different blood parameters were measured. Among the hematological parameters, there was sex wise difference in RBC count between males and females. Measurement of various types of blood cells was carried out with the help of a stage and an ocular micrometer. Erythrocytes were oval or elliptical shape both in male (length 15.375 ± 1.376 μm, breadth 10.75 ± 0.957 μm) and female (length 12.83 ± 3.083 μm, breadth 7.975 ± 2.206 μm) with centrally located oval nucleus (male: length 7.0 ± 0.816 μm, breadth 5.1 ± 0.115 μm; female: length 6.12 ± 0.853 μm, breadth 4 ± 0.816 μm). The WBCs was rounded, circular, or disk-shaped and the number of WBC in males was lower than females. The percentage of lymphocytes was higher in males and heterophils were lower in comparison with females but no differences in basophil, eosonophil, and monocyte counts in both the sexes during the study period. In addition, the content of hemoglobin percentage was higher in females than males. In terms of plasma chemistry, there was significant difference in the mean value of protein and cholesterol content between male (male, 98.264 ± 29.231and 7.455 ± 0.503 mg/ml; female, 49.448 ± 32.988 and 9.567 ± 4.363 mg/ml) and females.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Alleman AR, Jacopson ER, Raskins ER (1992) Morphologic and cytochemical characteristics of blood cells from the desert tortoise (Gopherus agassizii). Am J Vet Res 53:1645–1651

    PubMed  CAS  Google Scholar 

  • Alleman AR, Jacopson ER, Raskins ER (1999) Morphologic, cytochemical staining and ultrastructural characteristics of blood cells from eastern diamondback rattle snake (Crotalus adamantius). Am J Vet Res 60:507–514

    PubMed  CAS  Google Scholar 

  • Anderson NL (1992) Diseases of Iguana iguana. Compend Cont Educ Pract Vet 14:1335–1343

    Google Scholar 

  • Barten SL (1993) The medical care of iguanas and other common pet lizards. Vet Clin N Am 23:1213

    CAS  Google Scholar 

  • Bonadiman SF, Miranda Farlen JB, Ribeiro Maria LS, Rabelo G, Lainson R, Silva EO, DaMatta RA (2010) Hematological parameters of Ameiva ameiva (Reptilia: Teiidae) naturally infected with hemogregarine: confirmation of monocytosis. Vet Par 171:146–150

    Article  Google Scholar 

  • Campbell TW (1996) Clinical pathology. In: Mader DR (ed) Reptile Medicine and Surgery. WB Saunders, Philadelphia, pp 248–257

    Google Scholar 

  • Campbell TW (1998) Interpretation of the reptilian blood profile. Exotic Pet Practice 3:33–36

    Google Scholar 

  • Canfield PJ, Shea GM (1988) Morphological observations on the erythrocytes, leukocytes and thrombocytesof blue tongue lizards (Lacertilia: Scincidae, Tiliqua). Anat Histol Embryol 17:328–342

    Article  PubMed  CAS  Google Scholar 

  • Cannon MS, Freed DA, Freed PS (1996) The leukocytes of the rough tail gecko Cytrtopodion scabruas: a bright-field and phase-contrast study. Anat Histol Embryol 25:11–14.a

    Article  PubMed  CAS  Google Scholar 

  • Coulson RA, Hernandez T (1964) Biochemistry of the alligator. A study of metabolism in slow motion. State Univ. Press, Baton Rouge

    Google Scholar 

  • Cranfield M, Graczyk T, Lodwick L (1996) Adenovirus in the breaded dragon, Pogona vitticeps. In: Proc. of the Third Annual Conf. Ass. Rept. Amph. Vet, pp. 131–132

  • de Pienaar UV (1962) Hematology of some South African reptiles. Witwatersrand Univ. Press, Johannesburg

    Google Scholar 

  • Divers SJ, Redmayne G, Aves SK (1996) Hematological and biochemical values of 10 green iguanas (Iguana iguana). Vet Rec 138:203–205

    Article  PubMed  CAS  Google Scholar 

  • Duguy R (1970) Numbers of blood cells and their variations. In: Gans C, Parsons TS (eds) Biology of the Reptilia. Vol. 3. Morphology C. Acad. Press, London–New York, pp 93–104

    Google Scholar 

  • Efrati P, Nir E, Yaari A (1970) Morphological and cytochemical observations on cells of the hemopoietic system of Agama stellio. Israel J Med Sci 6:23–31

    PubMed  CAS  Google Scholar 

  • Engbretson GA, Hutchinson VH (1976) Erythrocyte count hematocrit and hemoglobin content in the lizard, Liolaemus multiformis. Copeia 1976:86

    Article  Google Scholar 

  • Frye FL (1991) Hematology as applied to clinical reptile medicine. In: Reptile Care. An Atlas of Diseases and Treatment, TFH Publication Inc., Neptune City (NJ, USA), pp. 211–277

  • Harr KE, Alleman AR, Dennis PM, Maxwell LK, Lock BA, Bennett RA, Jacobson ER (2001) Morphologic and cytochemical characteristics of blood cells and hematologic and plasma biochemical reference ranges in green iguanas. J Am Vet Med Assoc 218:915–921

    Article  PubMed  CAS  Google Scholar 

  • Hartman FA, Lessler MA (1964) Erythrocyte measurements in fishes, amphibians and reptiles. Boil Bull 126:83–88

    Article  Google Scholar 

  • Hawkey CM, Dennett TB (1989) A colour atlas of comparative veterinary hematology. Wolfe Medical Publications Ltd, Ipswich

    Google Scholar 

  • Ismail HU, Sevinc MS, Yildirimhan HS (2003) Erythrocyte size and morphology of some tortoises and turtles from Turkey. Zool Studies 42:173–178

    Google Scholar 

  • Izard Y, Detrait J, Boquet P (1961) Variations saisonnieres de la composition du sang de Vipera aspis. Ann Inst Pasteur Paris 100:539–545

    PubMed  CAS  Google Scholar 

  • Knotek Z, Hauptman K, Knotkova Z, Hajkova P, Ticht F (2002) Hemogram and plasma biochemistry in green iguanas with renal disease. Acta Vet Brno 71:333–340

    Article  Google Scholar 

  • Knotek Z, Knotkova Z, Doubek J, Pejrilova S, Hauptman K (2003) Plasma biochemistry in female green iguanas (Iguana iguana) with calcium metabolism disorders. Acta Vet Brno 72:183–189

    Article  CAS  Google Scholar 

  • Knotkova Z, Doubek J, Knotkova Z, Hajkova P (2002) Blood cell morphology and plasma biochemistry in Russian Tortoises (Agrionemys horsfieldi). Acta Vet Brno 71:191–198

    Article  CAS  Google Scholar 

  • Kohler G (1996) Krankheiten der amphibien and reptilien, Eugen Ulmer Verlag

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin-phenol reagent. J Biol Chem 193:265–275

    PubMed  CAS  Google Scholar 

  • Luck JM, Keller L (1929) The blood chemistry of two species of rattlesnakes Crotalus atrox and Crotalu soregonu. Ann Sci Nat 12:289–291

    Google Scholar 

  • Martinez Silvestre A, Rodriguez Dominguez MA, Mateo JA, Pastor J, Marco I, Lavin S, Cuenca R (2004) Comparative haematology and blood chemistry of endangered lizards (Gallotia species) in the Canary Islands. Vet Rec 155:266–269

    Article  PubMed  CAS  Google Scholar 

  • Mateo MR, Roberts ED, Enright FM (1984) Morphologic, cytochemical and functonal studies of peripheral blood cells from young healthy American alligator (Alligator mississippiensisis). Am J Vet Res 45:1046–1053

    PubMed  CAS  Google Scholar 

  • McArthur S (1996) Veterinary management of tortoises and turtles. Blackwell Science, Oxford

    Google Scholar 

  • Muro J, Cuenca R, Pastor J, Vinas L, Lavin S (1998) Effects of lithium heparin and tripotassium EDTA on hematologic valuses of Hermannes tortoises (Testudo hermanni). J Zool Wildlife Med 29:40–44

    CAS  Google Scholar 

  • Olufemi AS, Adeyinka AO (1994) Hematological values of the rainbow lizard Agama agama L. J Herpetol 4:86–90

    Google Scholar 

  • Pal A, Parida SP, Swain MM (2008) Hematological and plasma biochemistry in fan-throated lizard, Sitana ponticeriana (Sauria: Agamidae). Rus J Herp Rus 15(2):110–116

    Google Scholar 

  • Pejrilova S, Knotkova Z, Knotek Z, Varbas V (2004) Age-related changes of the hematological profile in green iguana (Iguana iguana rhinolopha). Acta Vet Brno 73:305–312

    Article  Google Scholar 

  • Puerta M, Abelenda M, Salvador A, Matrin J, Lopez P, Veiga JP (1996) Haematology and plasma chemistry of male lizards. Psammodromus algirus. Effects of testosterone treatment. Comp Haematol Int 6(2):123–124

    Article  Google Scholar 

  • Redrobe S, MacDonald J (1999) Sample collection and clinical pathology of reptiles. Vet Cli Am 2:709–730

    CAS  Google Scholar 

  • Rosenthal HL, Pfluke ML, Buscaglia S (1960) A stable iron reagent for determination of cholesterol. J Lab Clin Med 50:318

    Google Scholar 

  • Saint Girons M (1970) Morphology of the circulating blood cells. In: Gans C, Parsons TS (eds) Biology of the Reptilia. Vol. 3. Morphology C. Acad. Press, London–New York, pp 73–91

    Google Scholar 

  • Sevinc M, Ugurtas IH (2001) The morphology and size of blood cells of Lacerta rudis bithynica (Squamata: Reptilia). Tur Asiatic Herpetol Res 9:122–129

    Google Scholar 

  • Sevinc M, Ugurtas IH, Yildinmhan HS (2000) Erythrocytes measurements in Lacerta rudis (Reptilia: Lacertidae). Tur J Zool 24:207–209

    Google Scholar 

  • Sypek J, Borysenko M (1988) ÝReptiles. In: Rowley AF, Ratcliffe NA (eds) Vertebrate blood cells. Cambridge Univ. Press, Cambridge, pp 211–256

    Google Scholar 

  • Szarski H, Czopek G (1984) Erythrocyte diameter in some amphibians and reptiles. Bull Acad Sci Pol Ci IISer Biol 14:443–447

    Google Scholar 

  • Troiano JC, Gould EG, Gould I (2008) Hematological reference intervals in argentina lizard Tupinambis merianae (Sauria-Teiidae). Comp Clin Pathol 17:93–97

    Article  Google Scholar 

  • Walberg J (2001) White blood cells counting technique in birds. Semin Avian Exotic Pet Med 10:72–76

    Article  Google Scholar 

Download references

Acknowledgments

We are extremely grateful to the Head of Utkal University, Bhubaneswar, India, for providing the necessary facilities to carry out this work.

Author information

Authors and Affiliations

  1. Regional Museum of Natural History, Bhubaneswar, 751013, India

    Siba Prasad Parida

  2. North Orissa University, Baripada, 757003, India

    Sushil K. Dutta

  3. Molecular Biology Lab, School of Biotechnology, KIIT University, Bhubaneswar, 751024, India

    Arttatrana Pal

Authors
  1. Siba Prasad Parida
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sushil K. Dutta
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Arttatrana Pal
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Arttatrana Pal.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Parida, S.P., Dutta, S.K. & Pal, A. Hematological and plasma biochemistry in Psammophilus blanfordanus (Sauria: Agamidae). Comp Clin Pathol 21, 1387–1394 (2012). https://doi.org/10.1007/s00580-011-1303-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00580-011-1303-7

Keywords

Search

Navigation