Abstract
The influence of cadmium dust (containing lead, cadmium, copper, zinc, silicium and other elements) on acid phosphatase activity of Pisolithus arrhizus was observed by means of electron microscopy. Dust-treated mycelium showed increased activity of the enzyme, especially on the surface of the cell wall. There was an increase in abundance of autophagic vacuoles marked by a strong phosphatase reaction. An increase in the number of hyphae with diffuse enzyme activity within the cytoplasm coincided with a decrease of lifespan of the fungus, rapid changes in the mictoplasm stage, earlier closing of the dolipori and presumably the earlier autolysis of cell cytoplasm. Hyphae showing strong autolytic activity were separated from other hyphae by the material deposited within the doliporus and this whole area was devoid at that stage of acid phosphatase activity. The role of the enzyme in the mechanism of resistance to toxic elements is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Coulomb P, Coulomb C (1972) Processus d'autophagie cellulaire dans les cellules de meristemes radiculaires de la courge (Cucurbita pepa L. Cucurbitaceae) mis en etat d'anoxie. C R Acad Sci D 274:214–217
Dexheimer J, Aubert-Dufresne MP, Gerard J, Le Tacon F, Mousain D (1986) Etude de la localization ultrastructurale des activits phosphasiques acides dans deux types d'Ectomycorhizes: Pinus nigra nigricans/Hebeloma crustuliniforme et Pinus pinaster/Pisolithus tinctorius. Bull Soc Bot Fr 133:343–352
Essner E (1973) Phosphatases. In: Hayat M (ed) Electron microscopy of enzymes. Van Nostrand Reinholdt, New York, p 53
Fortin JA (1969) Les mycorhizes ectotrophes des artres forestiers. Nat Can 95:287–305
Gourp V, Pargney J-C (1991) Immunocytolocalization des phosphatases acides de Pisolithus tinctorius L. lors de sa confrontation avec le systeme recinaire de Pinus sylvestris (Pers.) Desv. Crypt Mycol 12:293–304
Greszta J, Braniewski S, Chrzanowska E, Nosek A, Chlodny J, Zwolinski J (1987) The influence of dusts from chosen industrial plants on particular links of forest ecosystems of the Niepolomice Forest. Ekol Pol 35:291–326
Hall JL, Hawes C (1991) Electron microscopy of plant cells. Academic Press, London, p 466
Lacaze B (1983) Localization cytochimique des activites phosphatasiques acides de champignons mycorrhiziens developpes sur milieu complet ou carence en phosphate. Can J Bot 61:1411–1414
Macaskie LE, Dean ACR (1984) Cadmium accumulation by a Citrobacter sp. J Gen Microbiol 130:53
Macaskie LE, Dean ACR (1990) Metal sequestering biochemicals. In: Volesky B (ed) Biosorption of heavy metals. CRC Press, Boca Raton Ann Arbor Boston, pp 199–248
Mesquita JF (1972) Ultrastructure de formations comparables aux vacuoles autophagiques dans les cellules des racines de l'Allium cepa et du Lupinus albus L. Cytologia 37:95–110
Turnau K (1991) The influence of cadmium dust on fungi in a Pino-Quercetum forest. Ekol Pol 39:39–57
Turnau K, Kottke I, Oberwinkler F (1993) Element localization in Paxillus involutus-Pinus sylvestris mycorrhizas using electron energy loss spectroscopy and imaging. Bot Acta 106:213–219
Turnau K, Kottke I, Dexheimer J, Botton B (1994) Element distribution in Pisolithus arrhizus mycelium treated with cadmium dust. Bot Ann 74:137–142
Villiers TA (1972) Cytological studies in dormancy. II. Pathological ageing changes during prolonged dormancy release. New Phytol 7:145–152
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Turnau, K., Dexheimer, J. Acid phosphatase activity in Pisolithus arrhizus mycelium treated with cadmium dust. Mycorrhiza 5, 205–211 (1995). https://doi.org/10.1007/BF00203339
Issue Date:
DOI: https://doi.org/10.1007/BF00203339