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Ectomycorrhizal fungal communities of Coccoloba uvifera (L.) L. mature trees and seedlings in the neotropical coastal forests of Guadeloupe (Lesser Antilles)

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Abstract

We studied belowground and aboveground diversity and distribution of ectomycorrhizal (EM) fungal species colonizing Coccoloba uvifera (L.) L. (seagrape) mature trees and seedlings naturally regenerating in four littoral forests of the Guadeloupe island (Lesser Antilles). We collected 546 sporocarps, 49 sclerotia, and morphotyped 26,722 root tips from mature trees and seedlings. Seven EM fungal species only were recovered among sporocarps (Cantharellus cinnabarinus, Amanita arenicola, Russula cremeolilacina, Inocybe littoralis, Inocybe xerophytica, Melanogaster sp., and Scleroderma bermudense) and one EM fungal species from sclerotia (Cenococcum geophilum). After internal transcribed spacer (ITS) sequencing, the EM root tips fell into 15 EM fungal taxa including 14 basidiomycetes and 1 ascomycete identified. Sporocarp survey only weakly reflected belowground assessment of the EM fungal community, although 5 fruiting species were found on roots. Seagrape seedlings and mature trees had very similar communities of EM fungi, dominated by S. bermudense, R. cremeolilacina, and two Thelephoraceae: shared species represented 93 % of the taxonomic EM fungal diversity and 74 % of the sampled EM root tips. Furthermore, some significant differences were observed between the frequencies of EM fungal taxa on mature trees and seedlings. The EM fungal community composition also varied between the four investigated sites. We discuss the reasons for such a species-poor community and the possible role of common mycorrhizal networks linking seagrape seedlings and mature trees in regeneration of coastal forests.

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Acknowledgments

This research was funded by The Region of Guadeloupe. Part of the sequencing work was done using the grant “Diversité des champignons mycorhiziens des plantes” (DivMyc, to M.-A. Selosse) from the network Bibliothèque Du Vivant funded by the CNRS, the Museum National d’HistoireNaturelle and the INRA. We thank Jean Chabrol for help with sampling and identification of sporocarps, Marie-Pierre Dubois and Mathieu Sauve for help with the molecular typing, David Marsh for English corrections, and two anonymous referees for their comments. S. Séne received grants from the Institut de Recherche pour le Développement (IRD), the Ministry of Education and Researchof Senegal and the World Federation of Scientists (WFS).

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Authors and Affiliations

  1. Laboratoire Commun de Microbiologie, IRD/UCAD/ISRA, BP 1386, Dakar, Sénégal

    Seynabou Séne, Cheikh Ndiaye, Abdala Gamby Diédhiou, Samba Ndao Sylla & Amadou Bâ

  2. Département Systématique et Evolution, UMR 7205 CNRS OSEB Muséum national d’Histoire naturelle, CP 5045 rue Buffon, 75005, Paris, France

    Seynabou Séne, Alexandre Geoffroy & Marc-André Selosse

  3. Laboratoire de Biologie et Physiologie Végétales, Faculté des Sciences Exactes et Naturelles, Université des Antilles et de la Guyane, BP 592, 97159, Pointe-à-Pitre, Guadeloupe, France

    Raymond Avril & Amadou Bâ

  4. Laboratoire des Symbioses Tropicales et Méditerranéennes, UMR113 INRA/AGRO-M/CIRAD/IRD/UM2-TA10/J, Campus International de Baillarguet, 34398, Montpellier cedex 5, France

    Clémence Chaintreuil & Amadou Bâ

  5. IRD, UMR 195 LEMAR (UBO/CNRS/IRD/Ifremer), BP 1386, CP 18524, Dakar, Sénégal

    Oumar Sadio

  6. Laboratoire des Sciences Végétales et Fongiques, Faculté des Sciences Pharmaceutiques et Biologiques, Université de Lille Nord de France, BP 83, 59006, Lille cedex, France

    Régis Courtecuisse

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  1. Seynabou Séne
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Correspondence to Amadou Bâ.

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Séne, S., Avril, R., Chaintreuil, C. et al. Ectomycorrhizal fungal communities of Coccoloba uvifera (L.) L. mature trees and seedlings in the neotropical coastal forests of Guadeloupe (Lesser Antilles). Mycorrhiza 25, 547–559 (2015). https://doi.org/10.1007/s00572-015-0633-8

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