Abstract
As atmospheric carbon dioxide (CO2) concentrations rise, one important mechanism by which plants can gain greater access to necessary soil nutrients is through greater investment in their mycorrhizal symbionts. In this study, we tested the hypotheses that (1) plants increase C allocation to ectomycorrhizal fungi (EMF) under elevated CO2 conditions, (2) N fertilization decreases C allocation to EMF, and (3) EMF activity at the site of symbiotic C and nutrient exchange is enhanced with CO2 enrichment. To test these hypotheses, we examined expression levels of Pinus taeda genes encoding monosaccharide transport (MST) and ammonium transport (AMT) proteins thought to be involved in symbiotic C and N movement, respectively, from mycorrhizal root tips exposed to CO2 and N fertilization. We also examined EMF ribosomal RNA expression (18S rRNA) to determine EMF activity. There was a trend toward lower relative MST expression with increased CO2. AMT expression levels showed no significant differences between control and treatment plots. EMF 18S rRNA expression was increased in CO2-enriched plots and there was a marginally significant positive interactive effect of CO2 and N fertilization on expression (p = 0.09 and 0.10, respectively). These results are consistent with greater C allocation to EMF and greater EMF metabolic activity under elevated CO2 conditions, although selective allocation of C to particular EMF species and greater fungal biomass on roots are plausible alternative hypotheses.
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Acknowledgments
This work was supported by a National Science Foundation Doctoral Dissertation Improvement Grant to JLP and WF Morris, National Science Foundation grants to RV and RB Jackson, and the Howard Hughes Precollege Program for summer support given to P Khatri. Department of Energy and Electric Power Research Institute and Brookhaven National Laboratory provide Duke Forest FACE site support and maintenance. We gratefully acknowledge P Khatri for laboratory assistance, TY James for assistance with primer design, and D Armaleo for assistance with gene expression methods and analyses. We thank JS Pippen and AC Oishi for providing access to soil respiration data. Many thanks to WF Morris, TY James, and two anonymous reviewers for their helpful comments on this manuscript.
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Parrent, J.L., Vilgalys, R. Expression of genes involved in symbiotic carbon and nitrogen transport in Pinus taeda mycorrhizal roots exposed to CO2 enrichment and nitrogen fertilization. Mycorrhiza 19, 469–479 (2009). https://doi.org/10.1007/s00572-009-0250-5
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DOI: https://doi.org/10.1007/s00572-009-0250-5