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NOD2/CARD15, NOD1/CARD4, and ICAM-1 gene polymorphisms in Turkish patients with inflammatory bowel disease

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Abstract

Purpose

The genetic susceptibility of people with certain NOD2/CARD15, NOD1/CARD4, and ICAM-1 gene variants to inflammatory bowel disease is still under investigation. The aim of this study was to investigate polymorphisms in the NOD2/CARD15 (R702W, G908R, and 3020insC), NOD1/CARD4 (E266K, D372N), and ICAM-1 (G241R, K469E) genes, which are known to be associated with inflammation, in Turkish patients with inflammatory bowel disease and healthy control groups.

Methods

The genotypes of 70 patients with endoscopically and histopathologically diagnosed Crohn's disease (38 men, 32 women; mean age, 38.8 ± 1.3), 120 patients with ulcerative colitis (67 men, 53 women; mean age, 41.7 ± 1.3) and 106 healthy control subjects (37 men, 69 women; mean age, 35.7 ± 1.4), who stated that they had never had any prior bowel disease history, were compared. A polymerase chain reaction-restriction fragment length polymorphism analysis was performed for two variants of the ICAM-1 gene, the three main variants of the NOD2/CARD15 gene, and the E266K variant of the NOD1/CARD4 gene, and DNA sequencing was used for the D372N polymorphism of the NOD1/CARD4 gene.

Results

In this study, the three previously described Crohn's disease-predisposing variants of the NOD2/CARD15 gene and the polymorphisms examined in the NOD1/CARD4 and ICAM-1 genes were not found to be associated with ulcerative colitis or Crohn's disease.

Conclusions

These findings suggest that the polymorphisms observed in the NOD2/CARD15, NOD1/CARD4, and ICAM-1 genes are not genetic susceptibility factors for Crohn's disease or ulcerative colitis in Turkey.

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Authors and Affiliations

  1. Institute of Biotechnology, Ankara University, Ankara, Turkey

    Senem Ceren Özen, Yasemin Çelik & A. Mithat Bozdayı

  2. Clinic of Gastroenterology, T.C. Yüksek İhtisas Hospital, Ankara, Turkey

    Ülkü Daǧlı, Mesut Yalin Kiliç & Aysel Ülker

  3. Department of Gastroenterology, School of Medicine, Ankara University, Cebeci, 06100, Ankara, Turkey

    Murat Törüner, Irfan Soykan, Hülya Çetinkaya, Ali Özden & A. Mithat Bozdayı

  4. Biometry Genetics Department, Faculty of Agriculture, Ankara University, Ankara, Turkey

    Muhip Özkan

  5. Institute of Hepatology, Ankara University, Ankara, Turkey

    A. Mithat Bozdayı

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  1. Senem Ceren Özen
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Özen, S., Daǧlı, Ü., Kiliç, M. et al. NOD2/CARD15, NOD1/CARD4, and ICAM-1 gene polymorphisms in Turkish patients with inflammatory bowel disease. J Gastroenterol 41, 304–310 (2006). https://doi.org/10.1007/s00535-005-1780-z

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