Abstract
To promote cytogenetical studies on cucumber (Cucumis sativus L., 2n = 2x = 14), the reciprocal crosses were made between autotriploid and diploid for selecting the primary trisomics. Meanwhile, chromosome behavior during meiosis in autotriploid cucumber was investigated to look for cytological evidences for origin of primary trisomics. Many viable F1 seeds were obtained from reciprocal crosses between autotriploid and diploid. The number of chromosomes of 56 surviving progenies varied from 14 to 28, with plants having 2n = 15 occurring at the highest frequency (51.8%). Primary trisomics were firstly obtained in this study. Four types of primary trisomics were isolated and they could be distinguished from each other, as well as diploid. Variable chromosome configurations, e.g. univalent, bivalents and trivalents were observed in many pollen mother cells of the autotriploid at metaphase I. Binomial chromosome distribution was observed at anaphase I and frequency of 8/13 was 6.25%. The meiosis of autotriploid, especially the class of gametes with eight chromosomes, gave the cytological evidence of producing 2x + 1 type gamete and could be induced into primary trisomic plants from progeny of autotriploid–diploid crosses. These studies have established a ground work for selecting a series of primary trisomics, and further using them for associating linkage groups with specific chromosomes in cucumber.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bradeen JM, Staub JE, Wye C, Antonise R (2001) Towards an expanded and integrated linkage map of cucumber (Cucumis sativus L.). Genome 44:111–119
Cao QH, Chen JF, Qian CT, Guo JY (2004) Cytological studies on meiosis and male gametophyte development in cucumber. Acta Bot Boreal Occident Sin 24(9):1721–1726
Carputo D, Barone A, Frusciante L (2000) 2n gametes in the potato: essential ingredients for breeding and germplasm transfer. Theor Appl Genet 101:805–813
Chen JF, Qian CT (2002) Studies on chromosome preparations using plant tendril as source tissue. Acta Hortic Sin 29(4):378–380
Chen JF, Staub JE, Qian CT et al (2003) Reproduction and cytogenetic characterization of interspecific hybrids derived from Cucumis hystrix Chakr × Cucumis sativus L. Theor Appl Genet 106:688–695
Costa Julia Y, Forni-Martins Eliana R (2004) A triploid cytotype of Echinodorus tennellus. Aquat Bot 79:325–332
Dane F (1991) Cytogenetics in genus Cucumis. In: Tsuchiya T, Gupta PK (eds) Chromosome engineering in plants: genetics, breeding, and evolution, part B. Elsevier, Amsterdam, pp 201–204
Dane F, Tsuchiya T (1976) Chromosome studies in the genus Cucumis. Euphytica 25:367–374
Fazio G, Staub JE, Stevens MR (2003) Genetic mapping and QTL analysis of horticultural traits in cucumber (Cucumis sativus L.) using recombinant inbred lines. Theor Appl Genet 107:864–874
Gao JH, Wang RQ, Mao LP, Diao XM (2003) Chromosome locating of dwarf gene in Foxtail Millet An’ai 3. Acta Agron Sin 29(1):152–154
Gaonkar RV, Tome SG (1991) Induced autotetraploidy in Ageratum conyzitdes L. Cytologia 56(3):327–331
Ishiki K (1991) Cytogenetical studies on African rice, Oryza glaberrima Steud. 3. Primary trisomics produced by pollinating autotriploid with diploid. Euphytica 55:7–13
Kennard WC, Poetter K, Dijkhuizen A, Meglic V, Staub JE, Havey MJ (1994) Linkages among RFLP, RAPD, isozyme, disease-resistance, and morphological markers in narrow and wide crosses of cucumber. Theor Appl Genet 89:42–48
Khazanehdari KA, Jones GH (1997) The causes and consequences of meiotic irregularity in the leek (Allium ampeloprasum spp. porrum); implication for fertility, quality and uniformity. Euphytica 93:313–319
Khush GS (1973) Cytogenetics of aneuploids. Academic Press, New York, p 301
Khush GS, Singh RJ, Sur SC, Librojo AL (1984) Primary trisomic of rice: origin, morphology, cytology, and use in linkage mapping. Genetica 107:141–163
Liu YQ (1994) A study on the pollen germination of some vegetables in cucurbitaceae. J Wuhan Bot Res 12(3):213–216
Mackiewicz HO, Malepszy S (1996) Obtaining and characterization of tetraploid forms in cucumber Cucumis sativus L. var. sativus and var. hardwickii Alef. Folia Hortic 8(1):3–10
Man H, Zhang CH, Liu LB, Shen SX, Sun ZW (2005) The triploid obtained from tetraploid by diploid crosses and its cytological studies in cabbage. J Plant Genet Resour 6(4):405–408
Ozaki Y, Narikiyo K, Fujita C, Okubo H (2004) Ploidy variation of progenies from intra- and inter-ploidy crosses with regard to trisomic production in asparagus (Asparagus officinalis L.). Sex Plant Reprod 17:157–164
Qian CT, Chen JF, Lou QF, Cao QH, Luo XD (2003) Studies on meiotic behaviors of pollen mother cells in cucumbers (Cucumis sativus L.). J Wuhan Bot Res 21(3):193–197
Romagosa I, Hecker RJ, Tsuchiya T, Lasa JM (1986) Primary trisomics in sugarbeet. I. Isolation and morphological characterization. Crop Sci 26:243–248
Satovic Z, Torres AM, Cubero JI (1998) Estimation of linkage in trisomic inheritance. Theor Appl Genet 96:513–518
Sung MP, Akira W, Michikazu H, Kenji U (2002) A tetraploid hybrid plant from 4x × 2x crosses in Vitis and its origin. Euphytica 126:345–353
Torres AM, Satovic Z, Canovas J, Cobos S, Cubero JI (1995) Genetics and mapping of new isozyme loci in Vicia faba L. using trisomics. Theor Appl Genet 91:783–789
Xie GS, Zhang DP, Xie YF (2001) Chromosomal localization of photoperiod sensitive genic male sterile genes using rice primary trisomics. J Wuhan Bot Res 19(3):255–258
Xu SJ, Singh RJ, Kollipara KP, Hymowitz T (2000) Primary trisomics in soybean: origin, identification, breeding behavior, and use in linkage mapping. Crop Sci 40:1543–1551
Yuan XJ, Pan JS, Cai R, Guan Y, Liu LZ, Zhang WW, Li Z, He HL, Zhang C, Si LT, Zhu LH (2008) Genetic mapping and QTL analysis of fruit and flower related traits in cucumber (Cucumis sativus L.) using recombinant inbred lines. Euphytica 164(2):473–491
Yutaka O, Takatoshi T (1997) Trisomic analysis of a strong photoperiod-sensitivity gene E1 in rice (Oryza sativa L.). Euphytica 95:301–307
Zhang HY, Ge FW, Wang YG, Xu Y, Chen QJ (2004) Construction of a reference linkage map for cucumber. Acta Hortic Sin 31(5):617–622
Zhang CH, Zhu HY, Li XF, Shen SX, Chen XP (2006) Obtaining and cytological identification of a set of primary trisomics in cabbage. Acta Hortic Sin 5(9):655–660
Zou JJ, Singh RJ, Lee J, Xu SJ, Cregan PB, Hymowitz T (2003) Assignment of molecular linkage groups to soybean chromosomes by primary trisomic. Theor Appl Genet 107:745–750
Acknowledgments
The authors wish to thank Mr. Ahmed Abbas Malik for his valuable suggestions about this manuscript. This research was supported by the General Program 30470120 and 30671419 from the National Natural Science Foundation of China; the ‘863’ Programs (2006AA10Z1A8, 2006AA100108) and the State Supporting Programs (2006BAD13B06, 2006BAD01A7-5-11) from the Ministry of Science and Technology of China.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by J.S. Heslop-Harrison.
Rights and permissions
About this article
Cite this article
Diao, WP., Bao, SY., Jiang, B. et al. Primary trisomics obtained from autotriploid by diploid reciprocal crosses in cucumber. Sex Plant Reprod 22, 45–51 (2009). https://doi.org/10.1007/s00497-008-0090-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00497-008-0090-z