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Isolation of S-RNase binding proteins from Solanum chacoense: identification of an SBP1 (RING finger protein) orthologue

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Abstract

Currently, the most attractive working model of gametophytic self-incompatibility (SI) involving S-RNases postulates the presence of an inhibitor protein or complex expressed in pollen tubes that would counteract the cytotoxic effect of the ribonuclease activity of the S-RNase. Since it has been previously shown that allele-specific recognition is mediated through the hypervariable domain sequence of the S-RNase, we have targeted this region to isolate pollen-expressed interacting proteins in the yeast two-hybrid system. One of the isolated proteins corresponds to a RING finger protein highly similar to the previously isolated SBP1 protein from Petunia hybrida. This protein is postulated to be part of the RING finger E3 ligase family. The ScSBP1 gene is expressed in almost all tissues tested, suggesting a more general role than only being involved in SI. Although the ScSBP1 gene is polymorphic, linkage analysis showed that it was unlinked to the S-locus. The isolation of this S-RNase-binding protein in two different species and with four different S-RNase sequences as bait, strengthens its putative involvement in the SI response. Furthermore, comparison of the bait sequences used suggests that the SBP1 protein interacts with conserved sequences located between the HVa and HVb domains.

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Acknowledgements

We would like to thank Gabriel Téodorescu for plant care and maintenance. This work was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC, Canada) and by Le Fond pour la Formation et l’Aide à la Recherche (FCAR-FQRNT, Québec).

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Authors and Affiliations

  1. Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, 4101 Sherbrooke est, Montreal, Quebec, H1X 2B2, Canada

    Martin O’Brien, Geneviève Major, Sier-Ching Chantha & Daniel P. Matton

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  1. Martin O’Brien
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  2. Geneviève Major
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  3. Sier-Ching Chantha
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  4. Daniel P. Matton
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Correspondence to Daniel P. Matton.

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Genbank accession numbers: ScSBP1, AY545464

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O’Brien, M., Major, G., Chantha, SC. et al. Isolation of S-RNase binding proteins from Solanum chacoense: identification of an SBP1 (RING finger protein) orthologue. Sex Plant Reprod 17, 81–87 (2004). https://doi.org/10.1007/s00497-004-0218-8

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  • DOI: https://doi.org/10.1007/s00497-004-0218-8

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