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Involvement of the ubiquitin/proteasome pathway in the organisation and polarised growth of kiwifruit pollen tubes

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Abstract

We recently reported the involvement of the ubiquitin pathway in microgametophyte development, and a direct role for the 26S proteasome in regulating pollen tube emergence in kiwifruit. Here we show that the ubiquitin/proteasome proteolytic pathway is involved not only in early kiwifruit pollen tube organisation, but also in maintaining polarised growth of tubes. By immunofluorescence analysis we show that ubiquitin and ubiquitin-protein conjugates are distributed mainly at the apex of emerging tubes, in both untreated pollen grains and pollen grains treated with MG132, an inhibitor of proteasome function. In the latter case, polysiphonous germination occurred and all the emerging areas were highly fluorescent. By adding MG132 to pollen when normal tube growth had already been established, accumulation of ubiquitin-protein conjugates, as well as a drastic reduction in tube growth and dramatic modifications of tube tip morphology were observed. Significantly, differential interference contrast microscopy analysis demonstrated that the clear zone was largely reduced or absent, and the nuclei were disconnected in their movements, reaching, in some cases, the extreme apex of the tip. These findings provide evidence that the ubiquitin- and proteasome-dependent proteolytic system could modulate the abundance and/or activity of key regulatory proteins involved in pollen tube emergence and polarised growth.

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Acknowledgement

We are grateful to Prof. Arthur L. Haas, Medical College of Wisconsin, Milwaukee, Wis., for providing the anti-ubiquitin antibody.

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Authors and Affiliations

  1. Istituto Botanico, Università di Urbino, via Bramante 28, 61029 , Urbino, Italy

    V. Scoccianti

  2. Dipartimento di Scienze Ambientali, Università della Tuscia, Viterbo, Italy

    E. Ovidi & A. Tiezzi

  3. Centro Interdipartimentale di Microscopia Elettronica, Università della Tuscia, Viterbo, Italy

    A. R. Taddei

  4. Istituto di Chimica Biologica, Università di Urbino, Urbino, Italy

    R. Crinelli & L. Gentilini

  5. Dipartimento di Biologia Evoluzionistica Sperimentale, Università di Bologna, Bologna, Italy

    A. Speranza

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  1. V. Scoccianti
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  2. E. Ovidi
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  3. A. R. Taddei
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  4. A. Tiezzi
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  5. R. Crinelli
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  6. L. Gentilini
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  7. A. Speranza
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Correspondence to V. Scoccianti.

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Scoccianti, V., Ovidi, E., Taddei, A.R. et al. Involvement of the ubiquitin/proteasome pathway in the organisation and polarised growth of kiwifruit pollen tubes. Sex Plant Reprod 16, 123–133 (2003). https://doi.org/10.1007/s00497-003-0182-8

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  • DOI: https://doi.org/10.1007/s00497-003-0182-8

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