Skip to main content
SpringerLink
Log in

Micropropagation of Campomanesia xanthocarpa O. Berg (Myrtaceae), a medicinal tree from the Brazilian Atlantic Forest

  • Original Article
  • Published:
Trees Aims and scope Submit manuscript

Abstract

Key message

Our study shows for the first time a complete guabiroba micropropagation system with acclimatized plants. BA was the best plant growth regulator for multiplication and IBA for rooting.

Abstract

Campomanesia xanthocarpa O. Berg (Myrtaceae), popularly known as guabiroba, is a woody species native to Brazil, important for its potential as a medicinal plant and fruit tree. For the first time, this study shows a complete system of micropropagation for this species. For this, nodal segments with two axillary buds each were used as starting material. Plant Preservative Mixture™ (0.1%) added to culture media was efficient in controlling contamination throughout the culture process. Two formulations of culture medium were compared for in vitro establishment. 2-Isopentenyladenine, 6-benzyladenine, kinetin and zeatin were tested during the multiplication step. Woody plant medium (WPM) was appropriate for all culture steps and, during the multiplication process, 2.2 µM BA induced the best numbers of new shoots per explant during three subcultures (3.0–3.5 per month). For rooting of microcuttings, a rate of 53% was reached in the WPM supplemented with 4.9 µM indol-3-butyric acid. A plastic box containing a mixture of commercial substrate and vermiculite (1:1 v/v) was used for plantlet acclimatization, allowing 52% of survival after two months. In conclusion, a complete micropropagation protocol was developed providing healthy plants. Further studies are needed to improve multiplication and survival rates.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Bates D, Maechler M, Bolker B, Walker S (2015) Fitting linear mixed-effects models using lme4. J Stat Softw 67:1–48

    Article  Google Scholar 

  • Biavatti MW, Farias C et al (2004) Preliminary studies on Campomanesia xanthocarpa (Berg.) and Cuphea carthagenensis (Jacq.) J. F. Macbr. aqueous extract: weight control and biochemical parameters. J Ethnopharmacol 93:385–389

    Article  CAS  PubMed  Google Scholar 

  • Blando F, Onlu S, Colella G, Konczak I (2013) Plant regeneration from immature seeds of Eugenia myrtifolia Sims. In Vitro Cell Dev Biol Plant 49:388–395

    Article  CAS  Google Scholar 

  • Brunner Scutti M (2000) Propagação vegetativa da guabirobeira (Campomanesia xanthocarpa Berg.) in vitro e por estaquia. Scientia Agraria 1(1):78–78

    Article  Google Scholar 

  • Cardoso CAL, Lima ASV, Ré-Poppi N, Vieira MC (2009) Fruit Oil of Campomanesia xanthocarpa O. Berg and Campomanesia adamantium O. Berg. J Essent Oil Res 21:481–483

    Article  CAS  Google Scholar 

  • Carnevali T, Vieira M, Luciano A, Gonçalves W, Rodrigues W, Ramos M (2015) Initial growth of Campomanesia xanthocarpa O. Berg under different substrate compositions. Rev Bras Plant Med 17:316–323

    Article  CAS  Google Scholar 

  • de Oliveira LS, Dias PC, Brondani GE (2013) Micropropagation of Brazilian forest species. Braz J For Res 33:439–453

    Google Scholar 

  • Dewir YH, Singh N, Mngomezulu S, Omar AMK (2011) Micropropagation and detection of important triterpenes in in vitro and field grown plants of Syzygium cordatum. J Med Plant Res 5:3078–3083

    CAS  Google Scholar 

  • Fox J, Weisberg S (2011) Functions and datasets to accompany. An R companion to applied regression, 2nd edn. Sage, Thousand Oaks

    Google Scholar 

  • Freire CG, Gardin JPP, Baratto CM, Vieira RL, Werner SS (2018) Micropropagation’s complete protocol of red araça (Psidium cattleianum, Myrtaceae) from germinated seeds in vitro. J Agr Sci 10:234–235

    Google Scholar 

  • George MW, Tripepi RR (2001) Plant preservative mixture™ can affect shoot regeneration from leaf explants of chrysanthemum, European birch and rhododendron. Hortic Sci 36:768–769

    Google Scholar 

  • George EF, Hall MA, Klerk GJD (2008) Plant growth regulators II: cytokinins, their analogues and antagonists. In: George EF, Hall MA, Klerk GJD (eds) Plant propagation by tissue culture. Springer, Dordrecht, pp 205–226

    Google Scholar 

  • Golle DP, Reiniger LRS, Curti AR, Léon EAB (2012) In vitro establishment and development of Eugenia involucrata DC.: influence of explant source and nutritional medium. Ciênc Flor 22:207–214

    Google Scholar 

  • Hothorn T, Bretz F et al. (2017) Package multcomp: simultaneous inference in general parametric models. R package version 3.2.5.pp : 1–128. https://cran.r-project.org/web/packages/multcomp/multcomp.pdf.

  • Huh SY, Lee KJ, Kim IJ, Kang GB, Lee KY (2015) Effect of biocide addition on plantlet growth and contamination occurrence during the in vitro culture of blueberry. J Plant Biotechnol 42:111–116

    Article  Google Scholar 

  • Khan VS, Hausman JF, Rao KR (1999) Clonal multiplication of Syzygium alternifolium (Wight) Walp, through mature nodal segments. Silvae Genet 48:45–50

    Google Scholar 

  • Klafke JZ, Silva MA, Panigas TF et al (2010) Effects of Campomanesia xanthocarpa on biochemical, hematological and oxidative stress parameters in hypercholesterolemic patients. J Ethnopharmacol 127:299–305

    Article  PubMed  Google Scholar 

  • Kumar S, Singh R, Kalia S, Sharma SK, Kalia R (2016) Recent advances in understanding the role of growth regulators in plant growth and development in vitro- I: Conventional growth regulators. Indian For 142(5):459–470

    Google Scholar 

  • Liu X, Yang G (2011) Clonal propagation of guava (Psidium guajava L.) on nodal explants of mature elite cultivar. Int Plant Biol 2(1):e2

    Article  Google Scholar 

  • Lloyd G, McCown B (1980) Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Proc Intern Plant Propagat Soc 30:421–427

    Google Scholar 

  • Machakova I, Zazimalova E, George EF (2008) Plant growth regulators I. Introduction: auxins, their analogues and inhibitors. In: George EF, Hall MA, Klerk GJD (eds) Plant propagation by tissue culture. Springer, Dordrecht, pp 175–204

    Google Scholar 

  • Markman BE, Bacchi EM, Kato ET (2004) Antiulcerogenic effects of Campomanesia xanthocarpa. J Ethnopharmacol 94:55–57

    Article  PubMed  Google Scholar 

  • Melchior SJ, Custódio CC, Marques TA, Neto NBM (2006) Colheita e armazenamento de sementes de gabiroba (Camponesia adamantium Camb.- Myrtaceae) e implicações na germinação. Rev Bras Sem 28:141–150

    Article  Google Scholar 

  • Mishra M, Chandra R, Pati R, Bajpai A (2007) Micropropagation of guava (Psidium guava L.). Acta Hortic 735:155–158

    Article  Google Scholar 

  • Miyazaki J, Tan BH, Errington SG (2010) Eradication of endophytic bacteria via treatment for axillary buds of Petunia hybrid using Plant Preservative Mixture (PPM™). Plant Cell Tissue Organ Cult 102:365–372

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nascimento AC, Paiva R, Abbade CL, Vargas PD, Soares FP (2008) Micropropagation of Eugenia pyriformis Cambess: effect of BAP and AIB. Rev Verde 3:20–26

    Google Scholar 

  • Oksanen J, Blanchet FG, Kindt R, Legendre P, Minchin PR, O’Hara RB (2016) Vegan: community ecology package. R package version 3.2.5. https://cran.r-project.org/web/packages/vegan/index.html.

  • Oltramari AC, Dal Vesco LL, Pedrotti EL, Ducroquet JHH, Nodari RO, Guerra MP (2000) Feijoa (Acca sellowiana (Berg) Burret) micropropagation protocol. Cienc Rural 30:61–68

    Article  Google Scholar 

  • Parra L, Amo-Marco JB (1996) Effect of plant growth regulators and basal media on in vitro shoot proliferation and rooting of Myrtus communis L. Biol Plant 38:161–168

    Article  CAS  Google Scholar 

  • Pinheiro J, Bates D, Debroy S, Sarkar D (2016) nlme: Linear and 745 Nonlinear mixed effects models. R package version 3.2.5. 1–128. https://cran.r-project.org/web/packages/nlme/index.htm.

  • Pospisilová J, Tichá I, Kadlecek P, Haisel D (1999) Acclimatization of micropropagated plants to ex vitro conditions. Biol Plant 42:481–497

    Article  Google Scholar 

  • Pospisilová J, Sinková H, Haisel D, Semoradová S (2007) Acclimatization of micropropagated plants to ex vitro conditions: effects of air humity, irradiance, CO2, concentration and abscisic acid: a review. Acta Hortic 74:29–38

    Article  Google Scholar 

  • Quoirin M, Lepoivre P (1977) Etude de milieux adaptés aux cultures in vitro de Prunus. Acta Hortic 78:437–442

    Article  Google Scholar 

  • Ross S, Grasso R (2010) In vitro propagation of ‘Guayabo del país’ (Acca sellowiana (Berg.) Burret). Fruit Veg Cereal Sci Biotech 4:83–87

    Google Scholar 

  • Rossato M, Schumacher PV, Netto APC, Souza GC, Reis EF, Stein CV (2015) Multiplication and in vitro rooting of Campomanesia adamantium Camb. Plant Cell Cult Micropropag 11:70–77

    Google Scholar 

  • Schmeda-Hirschmann G (1995) Flavonoids from Calycorectes, Campomanesia, Eugenia and Hexachlamys species. Fitoterapia 66:373–374

    CAS  Google Scholar 

  • Silva PRD, Rispoli RG, Minozzo MM, Jobim LH, Junges M, Stefenon VM (2014) A regenerative route for Eugenia uniflora L. (Myrtaceae) through in vitro germination and micropropagation. Ann For Res 57:39–45

    Google Scholar 

  • Sobral M, Proença C, Souza M, Mazine F, Lucas E (2016) Myrtaceae in Flora do Brasil 2020 em construção. Botanical Garden of Rio de Janeiro. https://floradobrasil.jbrj.gov.br/reflora/floradobrasil/FB171.

  • Souza JAD, Schuch MW, Donini LP, Ribeiro MDF (2008) Types and concentrations of cytokinin on in vitro multiplication of ‘pitangueira’. Cienc Rural 38(7):2046–2048

    Article  Google Scholar 

  • Souza LS, Fior CS, Souza PV, Schwarz SF (2011) Disinfestation of seeds and in vitro multiplication of guabijuzeiro from apical segments juveniles (Myrcianthes pungens O. Berg) D. Legrand. Rev Bras Frutic 33(3):691–697

    Article  Google Scholar 

  • Teleginski F, Zuffellato-Ribas KC, Koehler HS et al (2018) Vegetative propagation of Campomanesia xanthocarpa Mart. ex O Berg by air layering. Ciênc Florest 28:820–826

    Article  Google Scholar 

  • Viecili PRN, Borges DO, Kirsten K, Malheiros J, Vieceli E, Melo RD, Trevisan G, Silva MA, Bochi GV, Moresco RN, Klafke JZ (2014) Effects of Campomanesia xanthocarpa on inflammatory processes, oxidative stress, endothelial dysfunction and lipid biomarkers in hypercholesterolemic individuals. Atherosclerosis 234:85–92

    Article  CAS  PubMed  Google Scholar 

  • Vinagre AS, Ronnau AO, Pereira SF, Silveira LU, Willand EF, Suyenaga ES (2010) Anti-diabetic effects of Campomanesia xanthocarpa (Berg) leaf decoction. Braz J Pharmaceut Sci 46:169–177

    Article  Google Scholar 

Download references

Acknowledgements

The authors thank the Coordenação de Aperfeiçoamento de Pessoa de Nível Superior (CAPES, Brazil) for a grant to the first author, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) for research funding, Duane F. Lima for taxonomy identification and Eileen Bagyary for editing the manuscript.

Funding

This study was funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPQ), Brazil.

Author information

Authors and Affiliations

  1. Programa de Pós-Graduação em Botânica, Universidade Federal do Paraná, Av. Cel. Francisco H. dos Santos, Curitiba, PR, 81530-000, Brazil

    Jean Santos Machado, Fabiano Rodrigo Maia & Marguerite Quoirin

  2. Embrapa Florestas, Colombo, PR, 83411-000, Brazil

    Juliana Degenhardt

Authors
  1. Jean Santos Machado
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Juliana Degenhardt
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fabiano Rodrigo Maia
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Marguerite Quoirin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jean Santos Machado.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Communicated by Merkle.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Machado, J.S., Degenhardt, J., Maia, F.R. et al. Micropropagation of Campomanesia xanthocarpa O. Berg (Myrtaceae), a medicinal tree from the Brazilian Atlantic Forest. Trees 34, 791–799 (2020). https://doi.org/10.1007/s00468-020-01958-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00468-020-01958-z

Keywords

Search

Navigation