Abstract
Metasequoia glyptostroboides Hu et Cheng (dawn redwood), a known relict living fossil, is listed as a Class I rare and endangered tree in China. An efficient in vitro propagation protocol was established in this study to overcome the shortage of germplasm that cannot be compensated by conventional reproduction and as a way to preserve this tree. This objective was achieved by testing the effect of plant growth regulators (PGRs) on shoot propagation and root formation. Axillary shoots, which could be induced on PGR-free woody plant medium (WPM), were efficiently propagated on WPM supplemented with 16.0 µM zeatin and 2.5 µM indole-3-butyric acid. Improved rooting was possible on WPM supplemented with 0.05 µM α-naphthaleneacetic acid. Rooting was possible both in vitro in vermiculite-based PGR-free liquid culture and ex vitro in plastic bags containing perlite: vermiculite (1:1, v/v). This effective in vitro micropropagation protocol for M. glyptostroboides serves as a way to conserve this tree and provides an opportunity for mass production to alleviate strain on natural populations.
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All data generated or analyzed during this study are included in this published article and its supplementary information files.
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Funding
This work was supported by the National Key Research Plan of China (Grant Number: 2016YFC0503) and Guangdong Province Science and Technology Program (Number: 2015B020231008).
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XHZ, YL, SJZ, KLW, FZ, JATdS, and GHM designed the experiment and provided guidance for the study. YPX and SYC prepared samples for all analysis. BYG and MYN participated in the statistical analysis of physiological changes. YPX was also involved in all statistical analyses and co-wrote the manuscript. JATdS co-wrote the manuscript. All authors read and approved the manuscript.
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Communicated by Klimaszewska.
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Xiong, Y., Chen, S., Guo, B. et al. An efficient micropropagation protocol for Metasequoia glyptostroboides Hu et Cheng from shoot segments of 2-year-old trees. Trees 34, 307–313 (2020). https://doi.org/10.1007/s00468-019-01905-7
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DOI: https://doi.org/10.1007/s00468-019-01905-7