Abstract
Key message
Here, we isolated a NAC transcription factor PtoVNS11 from Populus tomentosa Carr. Molecular characterization showed that PtoVNS11 was involved in the regulation of secondary cell wall formation in poplar.
Abstract
NAC domain transcription factors are important regulators that activate the secondary wall biosynthesis in wood formation. In this study, we isolated a NAC domain transcription factor PtoVNS11 from Populus tomentosa Carr. PtoVNS11 shares high identity with SND1 of Arabidopsis thaliana (59.6 %) and PtrWND1B (97.9 %) of P. trichocarpa. Gene expression analyses showed that PtoVNS11 gene was mainly accumulated in xylem and stem of poplar. Further, GUS expression driven by the PtoVNS11 promoter was observed in vascular tissues of vegetative and reproductive organs in transgenic Arabidopsis. Promoter deletion analysis revealed that the fragment (−283 to −171) with the secondary wall NAC-binding element was required for tissue-specific expression of the PtoVNS11 gene. Subcellular localization analysis showed that PtoVNS11:GFP fusion protein was localized in the nucleus. Transgenic poplar plants carrying 35S:PtoVNS11 exhibited dwarf phenotypes with shorter internode as compared to wild-type plants. PtoVNS11 overexpression resulted in massive deposition of lignin and increased thickening of secondary walls in transgenic poplar. Transcription analysis showed that the expression levels of many wood-associated genes were up-regulated in transgenic plants overexpressing PtoVNS11. Taken together, our results indicate that PtoVNS11 may be involved in the regulation of the secondary wall biosynthesis during wood formation in poplar.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (31370672, 31171620), the National Key Project for Research on Transgenic Plant (2011ZX08010-003), 100 Talents Programme of The Chinese Academy of Sciences, the Natural Science Foundation Project of CQ CSTC (CSTC2013JJB8007), the program for New Century Excellent Talents in University (NCET-11-0700), and the Fundamental Research Funds for the Central Universities (XDJK2014a005).
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The authors declare that they have no conflict of interest.
Author contribution statement
Conceived and designed the experiments: LY ST KL. Performed the experiments: LY XZ WL. Analyzed the data: KL WL LY. Contributed reagents/materials/analysis tools: WL LY YF. Wrote the paper: KL LY ST.
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Communicated by F. Canovas.
L. Yang and Y. Hou contributed equally to this work.
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Yang, L., Hou, Y., Zhao, X. et al. Identification and characterization of a wood-associated NAC domain transcription factor PtoVNS11 from Populus tomentosa Carr.. Trees 29, 1091–1101 (2015). https://doi.org/10.1007/s00468-015-1188-1
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DOI: https://doi.org/10.1007/s00468-015-1188-1