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Constructing genetic linkage maps for Pinus elliottii var. elliottii and Pinus caribaea var. hondurensis using SRAP, SSR, EST and ISSR markers

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Abstract

A total of 122 F1 individuals from a single full-sib interspecific hybrid family crossed between Pinus elliottii var. elliottii (PEE) and P. caribaea var. hondurensis (PCH) were used to construct a detailed genetic linkage maps using four types of molecular markers: sequence-related amplified polymorphism (SRAP), microsatellite (SSR), expressed sequence tag polymorphism (ESTP) and inter-simple sequence repeat (ISSR). There were 381 SRAP, 108 SSR, 25 ESTP and 32 ISSR loci, segregating in the interspecific F1 hybrid individuals. Framework maps were constructed at a LOD score threshold of 4.0 using the JoinMap® 3.0. The map for the male parent (PCH) had 108 markers in 16 linkage groups (LGs), with a total length of 1,065.9 cM (Kosambi) and an average marker interval of 9.87 cM. The map for the female parent (PEE) contained 93 markers in 19 LGs, with a total length of 1,006.7 cM (Kosambi) and an average marker interval of 10.82 cM. The maps for PCH and PEE covered 56.5 and 70.3 % of their respective genomes. Based on the position of 36 loci segregating in both parents, 8 homologous LGs between PEE and PCH were identified.

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Acknowledgments

The authors thank J. Lin and X. Lai of the Longshan Forest Farm, Guangdong Academy of Forestry for assistance in collection of the samples. Drs. Harry Wu and Milos Ivkovich of CSIRO Plant Industry, Australia are thanked for valuable comments and English editing on an early draft.

The study reported here was financially supported by the National Natural Science Foundation of China (30671706).

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Authors and Affiliations

  1. Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Hui-xiao Yang, Tian-yi Liu, Chun-xin Liu & Shao-wei Huang

  2. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangzhou, 510642, People’s Republic of China

    Shao-wei Huang

  3. Sino-Forest (China) Investment Corporation Ltd., Room 2410-2411, Citic Plaza, Tianhe, Guangzhou, 510613, People’s Republic of China

    Rui Luo

  4. Guangdong Academy of Forestry, Guangzhou, 510520, People’s Republic of China

    Fen-cheng Zhao

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  1. Hui-xiao Yang
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  3. Fen-cheng Zhao
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  4. Tian-yi Liu
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  5. Chun-xin Liu
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Correspondence to Shao-wei Huang.

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Communicated by J. E. Carlson.

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Yang, Hx., Luo, R., Zhao, Fc. et al. Constructing genetic linkage maps for Pinus elliottii var. elliottii and Pinus caribaea var. hondurensis using SRAP, SSR, EST and ISSR markers. Trees 27, 1429–1442 (2013). https://doi.org/10.1007/s00468-013-0890-0

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