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Discrete versus continuous analysis of anatomical and δ13C variability in tree rings with intra-annual density fluctuations

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Abstract

Intra-annual density fluctuations (IADFs) are anomalies of tree rings where wood density is abruptly altered after sudden changes in environmental conditions. Their characterisation can provide information about the relationship between environmental factors and eco-physiological processes during tree growth. This paper reports about the variability of anatomical traits and stable carbon isotopic composition along tree rings as resulting from the application of two different methodological approaches: (a) the separation of each ring into different regions (earlywood, latewood and IADF) and the comparison of anatomical and isotopic parameters measured in those specific sectors and (b) the analysis of such features in continuum along ring width. Moreover, different parameters of vessels (i.e. ecd—equivalent circle diameter, elongation, sphericity and convexity of vessel lumen) were considered to identify those more appropriate for the representation of intra-annual anatomical variations. The analysis was conducted on Arbutus unedo L. growing on the Elba Island (Italy); tree rings of this species form IADFs with features clearly responsive to the environmental conditions experienced during plant growth. Results showed that the first approach, although more suitable to obtain data for subsequent statistical comparisons and for the calculation of correlations with environmental parameters, suffers from elements of subjectivity due to the size and position of the selected tree-ring regions. The in continuum method allows a clearer identification of the variation of tree-ring properties along ring width. Regarding anatomical parameters, shape indexes were not suitable indicators of intra-annual variability. The overall analysis suggested that using both methodologies in synergy helps to gain complete information and avoid misleading interpretations of IADFs in tree rings.

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Acknowledgments

The authors thank L. Nardella (Parco Nazionale dell`Arcipelago Toscano) and D. Giove (Comunità Montana dell`Arcipelago Toscano) for assistance in the field; M. Nötzli for assistance with the preparation of the microsections; W. Schoch and H. Gärtner for providing helpful suggestions during the laboratory phase of this project.

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Authors and Affiliations

  1. Department of Arboriculture, Botany and Plant Pathology, University of Naples Federico II, via Università 100, 80055, Portici (Naples), Italy

    Veronica De Micco & Giovanna Aronne

  2. Paleoenvironments and Chronoecology, Ecole Pratique des Hautes Etudes (PALECO-EPHE), Institut de Botanique, 34090, Montpellier, France

    Giovanna Battipaglia

  3. Centre for Bio-Archeology and Ecology (UMR5059), Institut de Botanique, University of Montpellier 2, 34090, Montpellier, France

    Giovanna Battipaglia

  4. WSL Swiss Federal Institute for Forest, Snow and Landscape Research, 8903, Birmensdorf, Switzerland

    Giovanna Battipaglia & Paolo Cherubini

  5. Max-Plank Institute for Biogeochemistry, Box 100164, 07701, Jena, Germany

    Willi A. Brand & Petra Linke

  6. Paul Scherrer Institute, 5232, Villigen, Switzerland

    Matthias Saurer

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  1. Veronica De Micco
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  2. Giovanna Battipaglia
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  3. Willi A. Brand
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  4. Petra Linke
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  5. Matthias Saurer
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  6. Giovanna Aronne
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  7. Paolo Cherubini
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Correspondence to Veronica De Micco.

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Communicated by S. Leavitt.

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De Micco, V., Battipaglia, G., Brand, W.A. et al. Discrete versus continuous analysis of anatomical and δ13C variability in tree rings with intra-annual density fluctuations. Trees 26, 513–524 (2012). https://doi.org/10.1007/s00468-011-0612-4

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