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Laminin and transforming growth factor beta-1 in children with vesicoureteric reflux

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Abstract

High-grade vesicoureteric reflux (VUR) promotes the development of renal nephropathy (RN) due to scar formation. This process involves transforming growth factor beta-1 (TGF beta1), which stimulates production of the extracellular matrix proteins, including laminin (LN). The aim of the study was to assess LN and TGF beta1 concentration according to VUR grade. The study group (1) consisted of 54 patients aged 6.23 ± 4.15 years with VUR, including: A, 19 with grade II; B, 19 with grade III; and C, 16 with grades IV or V reflux. The control group (2) contained 27 healthy patients aged 6.76 ± 4.02 years. LN and total TGF beta1 concentrations in serum and urine were determined by the immunoenzymatic (EIA) method. To assess total serum TGF beta1 levels, we used a solid-phase enzyme-linked immunosorbent assay (ELISA). Both serum and urinary levels of LN and TGF beta1 in VUR patients were higher compared with controls (p < 0.05). The highest urinary concentration of LN and TGF beta1 was found in subgroup C. A positive correlation was noted between urinary TGF beta1 and LN. Increased TGF-beta1 and LN levels in urine of high-grade VUR children suggests a potential role in fibrogenesis. Further trials are needed to investigate the role of serum and urinary LN level in VUR children.

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Authors and Affiliations

  1. 1st Department of Pediatrics, Medical University of Białystok, ul. Waszyngtona 17, 15-274, Białystok, Poland

    Anna Sabasiñska, Walentyna Zoch-Zwierz, Anna Wasilewska & Tadeusz Porowski

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  1. Anna Sabasiñska
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  2. Walentyna Zoch-Zwierz
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  3. Anna Wasilewska
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  4. Tadeusz Porowski
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Correspondence to Anna Wasilewska.

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Sabasiñska, A., Zoch-Zwierz, W., Wasilewska, A. et al. Laminin and transforming growth factor beta-1 in children with vesicoureteric reflux. Pediatr Nephrol 23, 769–774 (2008). https://doi.org/10.1007/s00467-007-0723-8

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