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Growth plate height of uremic rats is influenced by severity and duration of renal failure

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Abstract

To understand the changes induced by uremia in the epiphyseal growth plate, two studies were performed in young rats. In study 1, the morphological features of the tibial growth cartilage of stunted rats with different degrees of reduction of renal function were analyzed 2 weeks after nephrectomy and compared with control rats. There was a negative (r=−0.549, P<0.05) correlation between serum urea nitrogen (SUN) concentrations and longitudinal growth rate. The heights (mean±SEM) of growth cartilage (564±32 vs. 366±9 µm) and its hypertrophic zone (321±25 vs. 157±6 µm) were greater (P<0.05) in uremic than control rats and were highly and positively correlated (r=0.604, P<0.03 and r=0.706, P<0.01) with SUN levels. In study 2, the time course of growth plate alterations was investigated in uremic rats sacrificed 1 (NX-1), 2 (NX-2), and 4 weeks (NX-4) after nephrectomy compared with their corresponding control animals (C-1, C-2, C-4). Growth cartilage and hypertrophic zone heights were greater in NX-2 (533±60 and 264±32 µm) than in C-2 (345±10 and 131±11 µm), with no significant differences in the other groups. This report shows that enlargement of the growth plate and its hypertrophic stratum is greatly, although not exclusively, influenced by the severity and duration of renal insufficiency.

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Acknowledgements

Funding for this work was provided by grants PM 96–0110 from the Spanish Ministry of Science and Education, by grants FIS 97–0556, FIS 98/0611, FIS 00/0878, and FIS 00/0140 from the Spanish Ministry of Health, and by the Fundación Nutrición y Crecimiento. We are grateful to Teresa Usín and Vanessa Loredo for their expert technical assistance.

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Authors and Affiliations

  1. Facultad de Medicina e Instituto Universitario de Oncología del Principado de Asturias, Universidad de Oviedo, Oviedo, Spain

    Marta Fernández-Fuente, Fernando Santos, Eduardo Carbajo-Pérez, Julián Rodríguez & Enrique García

  2. Facultad de Medicina, Universidad de Oviedo, Oviedo, Spain

    Ana Weruaga, Benito Amil & Inés Molinos

  3. Hospital Central de Asturias, SESPA, Spain

    Fernando Santos, Julián Rodríguez & Inés Molinos

  4. Hospital Alvarez Buylla, SESPA, Spain

    Enrique García

  5. Área de Pediatría, Facultad de Medicina, C/ Julian Clavería, 6. 33006 Oviedo, Spain

    Fernando Santos

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  1. Marta Fernández-Fuente
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  2. Fernando Santos
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  3. Eduardo Carbajo-Pérez
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  4. Julián Rodríguez
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  5. Ana Weruaga
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  6. Benito Amil
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  7. Inés Molinos
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  8. Enrique García
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Correspondence to Fernando Santos.

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Fernández-Fuente, M., Santos, F., Carbajo-Pérez, E. et al. Growth plate height of uremic rats is influenced by severity and duration of renal failure. Pediatr Nephrol 19, 187–192 (2004). https://doi.org/10.1007/s00467-003-1326-7

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  • DOI: https://doi.org/10.1007/s00467-003-1326-7

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