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Isolation, identification and bioactivity analysis of an endophytic fungus isolated from Aloe vera collected from Asir desert, Saudi Arabia

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Abstract

Endophytic fungi isolated from desert plants are among the less known organisms with potentially valuable applications. The bioactivities of an endophytic fungus isolated from Aloe vera, a plant found in central regions of Asir desert, Saudi Arabia. Based on primary phytochemical screening, an efficient isolate was selected and identified according to the sequence analysis of the internal spacer regions ITS1, ITS4 and the 5.8S region as Preussia africana belonging to the family Sporormiaceae. The crude extract of this fungus was evaluated for its bioactivities. Under static conditions, the crude extract at a concentration of 500 μg/mL had a strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging rate of 87%, whereas a higher concentration (100 μg/mL) had an astounding wound healing effect (42.6% at 48 h) when compared to positive control. Moreover, the crude extract with a concentration of 50 μg/mL was active against almost all cancer cell lines such as HeLa (cervical cancer), Hep G2 (liver cancer), MCF-7 (breast cancer), A549 (lung cancer), LN-229 (glioblastoma), A-431 (skin cancer), and kidney cell line (HEK 293T). The results suggest that the endophytic fungus P. africana from A. vera has wide therapeutic applications against severe disease conditions.

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Acknowledgements

This project was funded by the National Plan for Science, Technology and Innovation (MAARIFAH), King Abdulaziz City for Science and Technology, Kingdom of Saudi Arabia, Award Number (13-BIO1030-02).

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Authors and Affiliations

  1. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia

    Fuad Ameen & Mohamed A. Yassin

  2. Department of Biological Sciences, University of Arkansas, Fayetteville, AR, 72701, USA

    Steven L. Stephenson

  3. Department of Plant Protection, College of Agriculture, King Saud University, Riyadh, Saudi Arabia

    Saleh AlNadhari

  4. Agricultural Research Center, Plant Pathology Research Institute, Giza, Egypt

    Mohamed A. Yassin

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  1. Fuad Ameen
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  2. Steven L. Stephenson
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  3. Saleh AlNadhari
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  4. Mohamed A. Yassin
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Correspondence to Fuad Ameen.

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Ameen, F., Stephenson, S.L., AlNadhari, S. et al. Isolation, identification and bioactivity analysis of an endophytic fungus isolated from Aloe vera collected from Asir desert, Saudi Arabia. Bioprocess Biosyst Eng 44, 1063–1070 (2021). https://doi.org/10.1007/s00449-020-02507-1

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  • DOI: https://doi.org/10.1007/s00449-020-02507-1

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