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Green and efficient biosynthesis of pectin-based copper nanoparticles and their antimicrobial activities

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Abstract

Herein, we reported a green biosynthesis method of copper nanoparticles (CuNPs) at microwave irradiation condition by using pectin as a stabilizer and ascorbic acid as a reducing agent. Under the optimum conditions, CuNPs1 and 2 were synthesized under microwave times 0 and 3 min, respectively. Transmission electron microscope and scanning electron microscope (SEM) tests showed that CuNPs1 and 2 had irregular polygon particles with average diameters of 61.9 ± 19.4 and 40.9 ± 13.6 nm, respectively. Zeta potentials of CuNPs1 and 2 were −45.2 and −48.7 mV, respectively. X-ray diffraction, Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and X-ray photoelectron spectroscopy techniques were used to characterize the properties of CuNPs. Furthermore, inhibition zone tests showed that CuNPs2 exhibited higher antimicrobial activities against Escherichia coli, Staphylococcus aureus, and Aspergillus japonicus than CuNPs1. The antibacterial activities were also studied by the bacterial growth kinetics in broth media, and CuNPs2 exhibited lower minimum bactericidal concentrations than CuNPs1.

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Acknowledgements

This study was financially supported by a grant from the Middle-aged and Young Teachers’ Basic Ability Promotion Project of Guangxi (No. 2018KY0257), the Research Program of Guangxi Specially-invited Experts (Ting Fa [2018] 39th), the Guangxi Key Laboratory of Electrochemical and Magneto-chemical Functional Materials (EMFM 20162204), and Guangxi Science and Technology Base and Specialized Talents (AD19110074). The authors appreciate Ms. Liao Li-qiong for editing the figures in this manuscript.

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Authors and Affiliations

  1. Hunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha, 410125, China

    Pei-jun Li, Dong-lin Su, Gao-yang Li & Yang Shan

  2. Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, China

    Pei-jun Li, Jin-ye Liang, Ying Huang, Jiang-juan Pan & Ming-fang Peng

  3. Longping Branch Graduate School, Central South University, Changsha, 410125, China

    Dong-lin Su & Yang Shan

  4. Hunan Key Laboratory of Fruits and Vegetables Storage, Processing and Quality Safety, Changsha, 410125, China

    Gao-yang Li

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  1. Pei-jun Li
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  2. Jin-ye Liang
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  3. Dong-lin Su
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  4. Ying Huang
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Li, Pj., Liang, Jy., Su, Dl. et al. Green and efficient biosynthesis of pectin-based copper nanoparticles and their antimicrobial activities. Bioprocess Biosyst Eng 43, 2017–2026 (2020). https://doi.org/10.1007/s00449-020-02390-w

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  • DOI: https://doi.org/10.1007/s00449-020-02390-w

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