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Butanol and butyric acid production from Saccharina japonica by Clostridium acetobutylicum and Clostridium tyrobutyricum with adaptive evolution

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A Correction to this article was published on 20 May 2019

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Abstract

Optimal conditions of hyper thermal (HT) acid hydrolysis of the Saccharina japonica was determined to a seaweed slurry content of 12% (w/v) and 144 mM H2SO4 at 160 °C for 10 min. Enzymatic saccharification was carried out at 50 °C and 150 rpm for 48 h using the three enzymes at concentrations of 16 U/mL. Celluclast 1.5 L showed the lowest half-velocity constant (Km) of 0.168 g/L, indicating a higher affinity for S. japonica hydrolysate. Pretreatment yielded a maximum monosaccharide concentration of 36.2 g/L and 45.7% conversion from total fermentable monosaccharides of 79.2 g/L with 120 g dry weight/L S. japonica slurry. High cell densities of Clostridium acetobutylicum and Clostridium tyrobutyricum were obtained using the retarding agents KH2PO4 (50 mM) and NaHCO3 (200 mM). Adaptive evolution facilitated the efficient use of mixed monosaccharides. Therefore, adaptive evolution and retarding agents can enhance the overall butanol and butyric acid yields from S. japonica.

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Change history

  • 20 May 2019

    Unfortunately, the author name was wrongly published as Pailin Sukwang.instead of Pailin Sukwong.

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Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1D1A1A09918683).

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Food and Bio-industry Research Center, Hankyong National University, Anseong, 17579, South Korea

    Chae Hun Ra

  2. Department of Biotechnology, Pukyong National University, Busan, 48513, South Korea

    In Yung Sunwoo, Trung Hau Nguyen, Pailin Sukwang, Phunlap Sirisuk, Gwi-Taek Jeong & Sung-Koo Kim

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  1. Chae Hun Ra
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  2. In Yung Sunwoo
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  3. Trung Hau Nguyen
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  4. Pailin Sukwang
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Correspondence to Sung-Koo Kim.

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Ra, C.H., Sunwoo, I.Y., Nguyen, T.H. et al. Butanol and butyric acid production from Saccharina japonica by Clostridium acetobutylicum and Clostridium tyrobutyricum with adaptive evolution. Bioprocess Biosyst Eng 42, 583–592 (2019). https://doi.org/10.1007/s00449-018-02063-9

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  • DOI: https://doi.org/10.1007/s00449-018-02063-9

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