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Preparation and characterization of a microencapsulated polyethylene glycol cross-linked polyhemoglobin

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Abstract

Many complications are associated to the therapeutic use of blood, among which are not only transfusion adverse events but also other issues such as lack of donors and high costs for collecting, testing, preserving, and distributing blood packages. Therefore, a clinically viable “blood substitute” is considered the holy grail of traumatology and may greatly benefit medicine. One of the most successful approaches to date is conjugating hemoglobin with polyethylene glycol (PEG). This conjugation aims mainly at overcoming free cell hemoglobin toxicity, which makes its use as oxygen carrier in pure form unfeasible. To improve PEG-hemoglobin conjugates feasibility, we propose applying dual functional PEG cross-linking hemoglobin molecules encapsulated by a protein carrier. The new oxygen carrier showed mean values of the hydrodynamic diameter, dispersity, and zeta potential of 1370 nm, 0.029 and −36 mV, respectively, evidencing the successful synthesis of PEG bis(N-succinimidyl succinate) and polyhemoglobin as well as the structuring of protein carrier.

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Acknowledgments

The financial support of both Coordination of High-Level Personnel Training (CAPES) (Bolsista da CAPES—Proc. nº BEX 3084-14-0) and FAPESP (process nº 2011/21933-0) is gratefully acknowledged. The authors also acknowledge CAPES for financial support of Prof. A. Converti as Special Visiting Researcher (Science without Borders Program, project n. 2609/2013).

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Authors and Affiliations

  1. Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580 B16, Cidade Universitária, São Paulo, SP, 05508-000, Brazil

    Marcos C. Knirsch, Marco Antonio Stephano & Bronislaw Polakiewicz

  2. Capes Foundation, Ministry of Education of Brazil, Brasilia, DF, 70.040-020, Brazil

    Marcos C. Knirsch

  3. Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Polytechnic School, Genoa University, Via Opera Pia 15, 16145, Genoa, Italy

    Filippo Dell’Anno & Attilio Converti

  4. Chemical Engineering Department, Polytechnic School, University of São Paulo, Av. Prof. Lineu Prestes 580 B18, Cidade Universitária, São Paulo, SP, 05508-000, Brazil

    Dennis Chicoma

  5. Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 580 B15, Cidade Universitária, São Paulo, SP, 05508-000, Brazil

    Nádia A. Bou-Chacra

  6. Vascular and Endovascular Surgery Unit, Research Laboratory of Experimental and Clinical Vascular Biology, DISC, Genoa University and IRCCS San Martino Hospital, Largo Rosanna Benzi, 16132, Genoa, Italy

    Domenico Palombo

Authors
  1. Marcos C. Knirsch
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  2. Filippo Dell’Anno
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  3. Dennis Chicoma
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  4. Marco Antonio Stephano
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  5. Nádia A. Bou-Chacra
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  6. Domenico Palombo
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  7. Attilio Converti
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  8. Bronislaw Polakiewicz
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Corresponding author

Correspondence to Attilio Converti.

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The authors declare no competing financial interest.

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Knirsch, M.C., Dell’Anno, F., Chicoma, D. et al. Preparation and characterization of a microencapsulated polyethylene glycol cross-linked polyhemoglobin. Bioprocess Biosyst Eng 38, 2263–2269 (2015). https://doi.org/10.1007/s00449-015-1463-y

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  • DOI: https://doi.org/10.1007/s00449-015-1463-y

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