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Enhancing bio-hydrogen production from sodium formate by hyperthermophilic archaeon, Thermococcus onnurineus NA1

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Abstract

Hyperthermophilic archaeon, Thermococcus onnurineus NA1 was reported to grow on formate producing hydrogen (H2). In this study, to sustain high H2 production rate and demonstrate the feasibility of mass production of H2, high cell density cultivation of T. onnurineus NA1 on sodium formate was employed under optimized conditions. From batch cultures, it was observed that the salinity of medium, significantly changed by the addition of formate salt and pH-adjusting agent, crucially affected cell growth and H2 production. With salinity carefully controlled between 3.7 and 4.6 %, 400 mM sodium formate was found to be an optimal initial concentration for maximizing cell growth-associated H2 production. Under optimal conditions, the repeated batch culture with cell recycling showed high cell density of OD600 of 1.7 in 3 and 30 L bioreactor, and the volumetric H2 production rate was enhanced up to 235.7 mmol L−1 h−1, which is one of the highest values reported to date.

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Acknowledgments

This work was supported by the KIOST in-house program (PE99212), and the Development of Biohydrogen Production Technology Using the Hyperthermophilic Archaea program of the Ministry of Oceans and Fisheries in the Republic of South Korea.

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Author notes

  1. Jung Ho Jeon

    Present address: Department of Biological Sciences, Myongji University, Yongin, 449-728, Republic of Korea

Authors and Affiliations

  1. Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon, 305-333, Republic of Korea

    Seung Seob Bae, Hyun Sook Lee, Jung-Hyun Lee, Sung Gyun Kang & Tae Wan Kim

  2. Korea Institute of Ocean Science and Technology, Ansan, 426-744, Republic of Korea

    Seung Seob Bae, Hyun Sook Lee, Jung Ho Jeon, Jung-Hyun Lee, Sung Gyun Kang & Tae Wan Kim

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  1. Seung Seob Bae
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  2. Hyun Sook Lee
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  3. Jung Ho Jeon
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  4. Jung-Hyun Lee
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Correspondence to Sung Gyun Kang or Tae Wan Kim.

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Bae, S.S., Lee, H.S., Jeon, J.H. et al. Enhancing bio-hydrogen production from sodium formate by hyperthermophilic archaeon, Thermococcus onnurineus NA1. Bioprocess Biosyst Eng 38, 989–993 (2015). https://doi.org/10.1007/s00449-014-1336-9

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  • DOI: https://doi.org/10.1007/s00449-014-1336-9

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