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l-Lactic acid production benefits from reduction of environmental osmotic stress through neutralizing agent combination

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Abstract

This paper hinged on the combination effect of two different neutralizing agents Ca(OH)2 and NH4OH on the production of l-lactic acid by Lactobacillus paracasei. Present study quantitatively indicated that environmental osmotic pressure (844–1,772 mOsm/kg) exerted minor influence on l-lactic acid production, but a critical level fell on approximately 3,000 mOsm/kg which restricted l-lactic acid production significantly. Once osmotic pressure exceeded 3,600 mOsm/kg, l-lactic acid production ran aground. A new and efficient neutralizing agent-adding strategy was established in this study to procure 2.21-fold enhancement (5.94 g/l/h) relative to previous productivity of l-lactic acid with NH4OH as neutralizing agent via batch cultivation. It was, therefore, speculated that inhibition effect in the late phase of the fermentation might be in large part attributed to the dramatic increase of environmental osmotic stress, other than cumulative effect of lactate concentration itself.

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Acknowledgments

The authors gratefully acknowledge the financial support of the National Key Basic Research Program of China (973 Program, 2013CB733600) and the National Major Scientific and Technological Special Project for “Significant Scientific Instrument and Equipment Development” (2012YQ150087). The National Science and Technology Support Program (2012BAI44G01), National High Technology Research and Development Program 863 (2012AA021201) are also acknowledged.

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P.O. Box 329, Shanghai, 200237, People’s Republic of China

    Xiwei Tian, Yonghong Wang, Ju Chu, Yingping Zhuang & Siliang Zhang

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  1. Xiwei Tian
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  2. Yonghong Wang
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  3. Ju Chu
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  4. Yingping Zhuang
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  5. Siliang Zhang
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Correspondence to Yonghong Wang or Ju Chu.

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Tian, X., Wang, Y., Chu, J. et al. l-Lactic acid production benefits from reduction of environmental osmotic stress through neutralizing agent combination. Bioprocess Biosyst Eng 37, 1917–1923 (2014). https://doi.org/10.1007/s00449-014-1166-9

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  • DOI: https://doi.org/10.1007/s00449-014-1166-9

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