Abstract
Immense interest has been devoted to the production of bulk chemicals from lignocellulose biomass. Diluted sulfuric acid treatment is currently one of the main pretreatment methods. However, the low total sugar concentration obtained via such pretreatment limits industrial fermentation systems that use lignocellulosic hydrolysate. Sugarcane bagasse hemicellulose hydrolysate is used as the carbon and nitrogen sources to achieve a green and economical production of succinic acid in this study. Sugarcane bagasse was ultrasonically pretreated for 40 min, with 43.9 g/L total sugar obtained after dilute acid hydrolysis. The total sugar concentration increased by 29.5 %. In a 3-L fermentor, using 30 g/L non-detoxified total sugar as the carbon source, succinic acid production increased to 23.7 g/L with a succinic acid yield of 79.0 % and a productivity of 0.99 g/L/h, and 60 % yeast extract in the medium could be reduced. Compared with the detoxified sugar preparation method, succinic acid production and yield were improved by 20.9 and 20.2 %, respectively.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 21076105), “973” Program of China (No. 2009CB724701), Innovation Scholars Climbing Program (SBK200910195), “863” Program of China (No. 2011AA02A203), National Natural Science Foundation of China (Grant No. 21106066), a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, Qing Lan Project of Jiangsu Province, Natural Science Foundation of the Higher Education Institutions of Jiangsu Province, China(11KJB530003), and Program for Changjiang Scholars and Innovative Research Team in University.
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Xi, Yl., Dai, Wy., Xu, R. et al. Ultrasonic pretreatment and acid hydrolysis of sugarcane bagasse for succinic acid production using Actinobacillus succinogenes . Bioprocess Biosyst Eng 36, 1779–1785 (2013). https://doi.org/10.1007/s00449-013-0953-z
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DOI: https://doi.org/10.1007/s00449-013-0953-z