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An ammonium sulfate sensitive endoxylanase produced by Streptomyces

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Abstract

Streptomyces sp. CSWu2 was newly isolated and identified from Korean soil. In culture medium, the strain produced a highly active endoxylanase (Xynwu2), which was purified to homogeneity by a single-step chromatography on Poros-HQ. The xylanase was ~38 kDa and its activity was maximal at 65 °C and pH 11.0. It was stable up to 60 °C and from pH 8.0 to 12.0, and its activity was slightly enhanced by nonionic detergents, but inhibited by EDTA, EGTA, and divalent metal ions. Intriguingly, Xynwu2 was highly sensitive to ammonium sulfate, but its completely suppressed activity was recovered by desalting out. Xynwu2 produced xylose and xylobiose as principal end products from xylan, suggesting an endoxylanase nature. Importantly, scanning electron microscopy showed Xynwu2 efficiently degraded corncobs, an agro-industrial waste material. We believe that Xynwu2 is a potential candidate for converting lignocellulosic waste material into simple sugars which could be used to produce bioethanol and other value-added products.

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Acknowledgments

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) (2010-0029178) and a grant from the Next-Generation BioGreen 21 Program (No. PJ008140), Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Department of Pharmacy, Chosun University, Gwangju, 501-759, Republic of Korea

    Jaya Ram Simkhada, Yun Hee Choi & Jin Cheol Yoo

  2. Department of Chemical and Biological Engineering, Korea University, Seoul, Republic of Korea

    Hah Young Yoo & Seung Wook Kim

  3. Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Chonnam National University, Gwangju, 500-757, Republic of Korea

    Don Hee Park

  4. Department of Alternative Medicine, Gwangju University, Gwangju, 503-703, Republic of Korea

    Hyo Jeong Lee

Authors
  1. Jaya Ram Simkhada
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  2. Hah Young Yoo
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  3. Don Hee Park
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  4. Yun Hee Choi
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  5. Hyo Jeong Lee
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  6. Seung Wook Kim
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  7. Jin Cheol Yoo
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Corresponding authors

Correspondence to Seung Wook Kim or Jin Cheol Yoo.

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Jaya Ram Simkhada and Hah Young Yoo contributed equally to this work.

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Simkhada, J.R., Yoo, H.Y., Park, D.H. et al. An ammonium sulfate sensitive endoxylanase produced by Streptomyces . Bioprocess Biosyst Eng 36, 819–825 (2013). https://doi.org/10.1007/s00449-013-0908-4

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  • DOI: https://doi.org/10.1007/s00449-013-0908-4

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