Skip to main content
SpringerLink
Log in

Application of solid-phase extraction to agar-supported fermentation

  • Original Paper
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

Agar-supported fermentation (Ag-SF), a variant of solid-state fermentation, has recently been improved by the development of a dedicated 2 m2 scale pilot facility, Platotex. We investigated the application of solid-phase extraction (SPE) to Ag-SF in order to increase yields and minimize the contamination of the extracts with agar constituents. The selection of the appropriate resin was conducted on liquid-state fermentation and Diaion HP-20 exhibited the highest recovery yield and selectivity for the metabolites of the model fungal strains Phomopsis sp. and Fusarium sp. SPE applied to Ag-SF resulted in a particular compartmentalization of the culture. The mycelium that requires oxygen to grow migrates to the top layer and formed a thick biofilm. The resin beads intercalate between the agar surface and the mycelium layer, and trap directly the compounds secreted by the mycelium through a “solid–solid extraction” (SSE) process. The resin/mycelium layer is easily recovered by scraping the surface and the target metabolites extracted by methanol. Ag-SF associated to SSE represents an ideal compromise for the production of bioactive secondary metabolites with limited economic and environmental impact.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Berrueta LA, Gallo B, Vicente F (1995) Chromatographia 40:474–483

    Article  CAS  Google Scholar 

  2. Capriotti AL, Cavaliere C, Giansanti P, Gubbiotti R, Samperi R, Lagana A (2010) J Chromatogr A 1217:2521–2532

    Article  CAS  Google Scholar 

  3. Fritz JS, Macka M (2000) J Chromatogr A 902:137–166

    Article  CAS  Google Scholar 

  4. Hennion MC (1999) J Chromatogr A 856:3–54

    Article  CAS  Google Scholar 

  5. Pichon V (2000) J Chromatogr A 885:195–215

    Article  CAS  Google Scholar 

  6. Pico Y, Fernandez M, Ruiz MJ, Font G (2007) J Biochem Biophys Methods 70:117–131

    Article  CAS  Google Scholar 

  7. Degel F (1996) Clin Biochem 29:529–540

    Article  CAS  Google Scholar 

  8. Månsson M, Phipps RK, Gram L, Munro MHG, Larsen TO, Nielsen KF (2010) J Nat Prod 73:1126–1132

    Article  Google Scholar 

  9. Lee JC, Park HR, Park DJ, Lee HB, Kim YB, Kim CJ (2003) Lett Appl Microbiol 37:196–200

    Article  CAS  Google Scholar 

  10. Tolonen M, Saris PE, Siika-Aho M (2004) Appl Microbiol Biotechnol 63:659–665

    Article  CAS  Google Scholar 

  11. Rossi DT, Zhang N (2000) J Chromatogr A 885:97–113

    Article  CAS  Google Scholar 

  12. Tubitak OO (1986) J Appl Polym Sci 32:5533–5542

    Article  Google Scholar 

  13. Casey JT, Walsh PK, O’Shea DG (2007) Sep Purif Technol 53:281–288

    Article  CAS  Google Scholar 

  14. Lam KS, Veitch JA, Lowe SE, Forenza S (1995) J Ind Microbiol 15:453–456

    Article  CAS  Google Scholar 

  15. Xie Y, Van de Sandt E, De Weerd T, Wang NH (2001) J Chromatogr A 908:273–291

    Article  CAS  Google Scholar 

  16. Adelin E, Slimani N, Cortial S, Schmitz-Afonso I, Ouazzani J (2011) J Ind Microbiol Biotechnol 38(2):299–305

    Article  CAS  Google Scholar 

  17. Adelin E, Servy C, Cortial S, Lévaique H, Martin MT, Retailleau P, Le Goff G, Bussaban B, Lumyong S, Ouazzani J (2011) Phytochemistry 72(18):2406–2412

    Article  CAS  Google Scholar 

  18. Meca G, Ruiz MJ, Soriano JM, Ritieni A, Moretti A, Font G, Manes J (2010) Toxicon 56:418–424

    Article  CAS  Google Scholar 

  19. Tatum JH, Baker RA, Berry RE (1985) Phytochemistry 24:457–459

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors are grateful to Dr. Boonsom Bussaban and Pr. Saisamorn Lumyong, from Chiang Mai University, Thailand, for providing the fungal strains. This research was supported by a scholarship grant from the Institut de Chimie des Substances Naturelles, ICSN-CNRS.

Author information

Authors and Affiliations

  1. Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (C.N.R.S), Avenue de la Terrasse, 91198, Gif-sur-Yvette Cedex, France

    Géraldine Le Goff, Emilie Adelin, Sylvie Cortial, Claudine Servy & Jamal Ouazzani

Authors
  1. Géraldine Le Goff
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Emilie Adelin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sylvie Cortial
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Claudine Servy
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jamal Ouazzani
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jamal Ouazzani.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Le Goff, G., Adelin, E., Cortial, S. et al. Application of solid-phase extraction to agar-supported fermentation. Bioprocess Biosyst Eng 36, 1285–1290 (2013). https://doi.org/10.1007/s00449-012-0873-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-012-0873-3

Keywords

Search

Navigation