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Enzymatic synthesis of biodiesel from palm oil assisted by microwave irradiation

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Abstract

Optimal conditions for enzymatic synthesis of biodiesel from palm oil and ethanol were determined with lipase from Pseudomonas fluorescens immobilized on epoxy polysiloxane–polyvinyl alcohol hybrid composite under a microwave heating system. The main goal was to reduce the reaction time preliminarily established by a process of conventional heating. A full factorial design assessed the influence of ethanol-to-palm oil (8:1–16:1) molar ratio and temperature (43–57 °C) on the transesterification yield. Microwave irradiations varying from 8 to 15 W were set up according to reaction temperature. Under optimal conditions (8:1 ethanol-to-oil molar ratio at 43 °C), 97.56 % of the fatty acids present in the palm oil were converted into ethyl esters in a 12-h reaction, corresponding to a productivity of 64.2 mg ethyl esters g−1 h−1. This represents a sixfold increase from the process carried out under conventional heating, thus proving to be a potential tool for enhancing biochemical modification of oils and fats. In general, advantages of the new process include: (1) microwaves speed up the enzyme-catalyzed reactions; (2) there are no destructive effects on the enzyme properties, such as stability and substrate specificity, and (3) the microwave assistance allows the entire reaction volume to be heated uniformly. These bring benefits of a low energy demand and a faster conversion of palm oil into biodiesel.

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References

  1. Kubrakova IV, Toropchenova ES (2008) Microwave heating for enhancing efficiency of analytical operations (review). Inorg Mater 44:1509–1519

    Article  CAS  Google Scholar 

  2. Rejasse B, Lamare S, Legoy MD, Besson T (2007) Influence of microwave irradiation on enzymatic properties: applications in enzyme chemistry. J Enzym Inhib Med Chem 22:518–526

    Article  CAS  Google Scholar 

  3. Bassyouni FA, Abu-Bakr SM, Rehim MA (2012) Evolution of microwave irradiation and its application in green chemistry and biosciences. Res Chem Intermed 38:283–322

    Article  CAS  Google Scholar 

  4. Lindstrom P, Tierney J, Wathey B, Westman J (2001) Microwave assisted organic synthesis—a review. Tethahedron 57:9255–9283

    Google Scholar 

  5. Oghbaei M, Mirzaee O (2010) Microwave versus conventional sintering: a review of fundamentals, advantages and applications. J Alloy Compd 494:175–189

    Article  CAS  Google Scholar 

  6. Gupta MN, Roy I (2004) Enzymes in organic media: forms, functions and applications. Eur J Biochem 271:2575–2583

    Article  CAS  Google Scholar 

  7. Da Rós PCM, de Castro HF, Carvalho AF, Soares CMF, Moraes FF, Zanin GM (2012) Microwave-assisted enzymatic synthesis of beef tallow biodiesel. J Ind Microbiol Biotechnol 39:529–536

    Article  Google Scholar 

  8. Gupta M, Paul S, Gupta R (2009) General characteristics and applications of microwaves in organic synthesis. Acta Chim Sloven 56:749–764

    CAS  Google Scholar 

  9. Leadbeater NE, Stencel LM, Wood EC (2007) Probing the effects of microwave irradiation on enzyme-catalysed organic transformations: the case of lipase-catalysed transesterification reactions. Org Biomol Chem 5:1052–1055

    Article  CAS  Google Scholar 

  10. Wan H, Sun S, Hu X, Xia Y (2012) Nonthermal effect of microwave irradiation in nonaqueous enzymatic esterification. Appl Biochem Biotechnol 166:1454–1462

    Article  CAS  Google Scholar 

  11. Costa ICR, Leite SGF, Leal ICR, Miranda LSM, de Souza ROMA (2011) Thermal effect on the microwave assisted biodiesel synthesis catalyzed by lipases. J Braz Chem Soc 22:1993–1998

    Article  CAS  Google Scholar 

  12. Yu D, Wang Z, Chen P, Jin L, Cheng Y, Zhou J, Cao S (2007) Microwave-assisted resolution of (R, S)-2-octanol by enzymatic transesterification. J Mol Catal 48:51–57

    Article  CAS  Google Scholar 

  13. Fang Y, Huang W, Xia Y (2008) Consecutive microwave irradiation induced substrate inhibition on the enzymatic esterification. Process Biochem 43:306–310

    Article  CAS  Google Scholar 

  14. Hasan F, Shah AA, Hameed A (2006) Industrial applications of microbial lipases. Enzyme Microb Tech 39:235–251

    Article  CAS  Google Scholar 

  15. Rufino AR, Biaggio FC, Santos JC, de Castro HF (2009) Chemoenzymatic synthesis: a strategy to obtain xylitol monoesters. J Chem Technol Biotechnol 84:957–960

    Article  CAS  Google Scholar 

  16. Lee M, Lee D, Cho JK, Cho J, Han J, Park C, Kim S (2012) Improved high-pressure enzymatic batch synthesis in near-critical carbon dioxide. Bioprocess Biosyst Eng 35:105–113

    Article  CAS  Google Scholar 

  17. Freitas L, Da Ros PCM, Santos JC, De Castro HF (2009) An integrated approach to produce biodiesel and monoglycerides by enzymatic interestification of babassu oil (Orbinya sp). Process Biochem 44:1068–1074

    Article  CAS  Google Scholar 

  18. Li N, Zong M, Wu H (2009) Highly efficient transformation of waste oil to biodiesel by immobilized lipase from Penicillium expansum. Process Biochem 44:685–688

    Article  CAS  Google Scholar 

  19. Shah S, Gupta MN (2007) Lipase catalyzed preparation of biodiesel from Jatropha oil in a solvent free system. Process Biochem 42:409–414

    Article  CAS  Google Scholar 

  20. Moreira ABR, Perez VH, Zanin GM, De Castro HF (2007) Biodiesel synthesis by enzymatic transesterification of palm oil with ethanol using lipases from several sources immobilized on silica-PVA composite. Energ Fuel 21:3689–3694

    Article  CAS  Google Scholar 

  21. Urioste D, Castro MBA, Biaggio FC, De Castro HF (2008) Synthesis of chromatographic standards and establishment of a method for the quantification of the fatty ester composition of biodiesel from babassu oil. Quim Nova 31:407–412

    Article  CAS  Google Scholar 

  22. Santos JC, Paula AV, Nunes GFM, De Castro HF (2008) Pseudomonas fluorescens lipase immobilization on polysiloxane-polyvinyl alcohol composite chemically modified with epichlorohydrin. J Mol Catal B-Enzym 52–3:49–57

    Article  Google Scholar 

  23. Soares CMF, Castro HF, Moraes FF, Zanin GM (1999) Characterization and utilization of Candida rugosa lipase immobilized on controlled pore silica. Appl Biochem Biotech 77–9:745–757

    Article  Google Scholar 

  24. Polizzi KM, Bommarius AS, Broering JM, Chaparro-Riggers JF (2007) Stability of biocatalysts. Curr Opin Chem Biol 11:220–225

    Article  CAS  Google Scholar 

  25. Réjasse B, Lamare S, Legoy MD, Besson T (2004) Stability improvement of immobilized Candida antarctica lipase B in an organic medium under microwave radiation. Org Biomol Chem 2:1086–1089

    Article  Google Scholar 

  26. Schubert H, Regier M (2005) The microwave processing of foods. CRC Press, Taylor and Francis, ISBN-13: 978 1 85573 964 2

  27. Nelson SO (1978) Frequency and moisture dependence of dielectric properties of high- moisture corn. J Microwave Power EE 13:213–218

    Google Scholar 

  28. Venkatesh MS, Raghavan GSV (2004) An overview of microwave processing and dielectric properties of agri-food materials. Biosystems Eng 88:1–18

    Article  Google Scholar 

  29. Hayes BL (2002) Microwave synthesis: chemistry at the speed of light. CEM Publishing, Matthews, p 296

    Google Scholar 

  30. Terigar BG, Balasubramanian S, Boldor D (2010) An analysis of the microwave dielectric properties of solvent-oil feedstock mixtures at 300–3000 MHz. Bioresource Technol 101:6510–6516

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors are grateful for the financial support provided by CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) and FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) Brazil.

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Authors and Affiliations

  1. Engineering School of Lorena, University of São Paulo, 116, Lorena, São Paulo, 12602-810, Brazil

    Patrícia C. M. Da Rós, Larissa Freitas & Heizir F. de Castro

  2. State University of the North Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Av. Alberto Lamego no 2000, 28013-602, Brazil

    Victor H. Perez

Authors
  1. Patrícia C. M. Da Rós
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  2. Larissa Freitas
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  3. Victor H. Perez
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  4. Heizir F. de Castro
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Correspondence to Heizir F. de Castro.

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Da Rós, P.C.M., Freitas, L., Perez, V.H. et al. Enzymatic synthesis of biodiesel from palm oil assisted by microwave irradiation. Bioprocess Biosyst Eng 36, 443–451 (2013). https://doi.org/10.1007/s00449-012-0801-6

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  • DOI: https://doi.org/10.1007/s00449-012-0801-6

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