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Effect of compressed fluids treatment on β-galactosidase activity and stability

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Abstract

This study aimed at assessing the influence of different pressurized fluids treatment on the enzymatic activity and stability of a lyophilized β-galactosidase. The effects of system pressure, exposure time and depressurization rate, using propane, n-butane, carbon dioxide and liquefied petroleum gas on the enzymatic activity were evaluated. The β-galactosidase activity changed significantly depending on the experimental conditions investigated, allowing the selection of the proper compressed fluid for advantageous application of this biocatalyst in enzymatic reactions. The residual activity ranged from 32.1 to 93.8 % after treatment. The storage stability of the enzyme after high-pressure pre-treatment was also monitored, and results showed that the biocatalyst activity presents strong dependence of the fluid used in the pretreatment. The activity gradually decreases over the time for the enzyme treated with LGP and propane, while the enzyme treated with n-butane maintained 96 % of its initial activity until 120 days. For CO2, there was a reduction of around 40 % in the initial activity 90 days of storage. The enzyme treated with n-butane also showed a better thermostability in terms of enzymatic half-life.

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Acknowledgments

The authors thank FAPERGS, CAPES and CNPq for the financial support of this work and scholarships.

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Authors and Affiliations

  1. Chemistry and Food School, Federal University of Rio Grande-FURG, P.O. Box 474, Rio Grande, RS, CEP 96201900, Brazil

    Anna Rafaela Cavalcante Braga, Helen Treichel & Susana Juliano Kalil

  2. Department of Food Engineering, URI-Campus de Erechim, P.O. Box 743, Erechim, RS, CEP 99700-000, Brazil

    Marceli Fernandes Silva

  3. Department of Chemistry and Food Engineering, Federal University of Santa Catarina, UFSC, Campus Universitário, Bairro Trindade, P.O. Box 476, Florianópolis, SC, CEP 88040-900, Brazil

    J. Vladimir Oliveira

  4. Federal University of Fronteira Sul, Campus de Erechim, Av. Dom João Hoffmann, 313, Erechim, RS, CEP 99700-000, Brazil

    Helen Treichel

Authors
  1. Anna Rafaela Cavalcante Braga
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  2. Marceli Fernandes Silva
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  3. J. Vladimir Oliveira
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  4. Helen Treichel
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  5. Susana Juliano Kalil
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Corresponding authors

Correspondence to J. Vladimir Oliveira or Susana Juliano Kalil.

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Braga, A.R.C., Silva, M.F., Vladimir Oliveira, J. et al. Effect of compressed fluids treatment on β-galactosidase activity and stability. Bioprocess Biosyst Eng 35, 1541–1547 (2012). https://doi.org/10.1007/s00449-012-0744-y

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  • DOI: https://doi.org/10.1007/s00449-012-0744-y

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