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Assessment of process variables on 2-ethylhexyl palmitate production using Novozym 435 as catalyst in a solvent-free system

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Abstract

This work reports the optimization of 2-ethylhexyl palmitate production by esterification reaction in a solvent-free system using a commercial lipase as catalyst. For this, a sequential strategy was performed applying three experimental designs. An empirical model was built so as to assess the effects of process variables on the reaction conversion. Afterward, the operating conditions that optimized 2-ethylhexyl palmitate production were determined to be acid to alcohol molar ratio of 1:5.5, 70 °C, 150 rpm and 10.5 wt% of enzyme, leading to a reaction conversion as high as 93%. From this point, a kinetic study was carried out evaluating the influence of acid to alcohol molar ratio, enzyme concentration and temperature on product yield. Results obtained in this step allow to conclude that an excess of alcohol (acid to alcohol molar ratio of 1:6), relatively low enzyme concentration (10 wt%) and temperature of 70 °C led to nearly complete reaction conversion.

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Acknowledgments

The authors thank AGROPALMA, CAPES and CNPq for the financial support of this work and scholarships.

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Authors and Affiliations

  1. Escola de Química, EQ/UFRJ, CT, Bloco E, Rio de Janeiro, RJ, 21945-900, Brazil

    Aline Richetti & Selma G. F. Leite

  2. Instituto de Química, IQ/UFRJ, CT, Bloco A, Rio de Janeiro, RJ, 21945-900, Brazil

    Octávio A. C. Antunes & Lindomar A. Lerin

  3. Universidade Regional Integrada do Alto Uruguai e das Missões, Campus de Erechim, Av. Sete de Setembro, 1621, Erechim, RS, 99700-000, Brazil

    Rogério M. Dallago, Daniel Emmerich, Marco Di Luccio, J. Vladimir Oliveira, Helen Treichel & Débora de Oliveira

Authors
  1. Aline Richetti
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  2. Selma G. F. Leite
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  3. Octávio A. C. Antunes
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  4. Lindomar A. Lerin
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  5. Rogério M. Dallago
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  6. Daniel Emmerich
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  7. Marco Di Luccio
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  8. J. Vladimir Oliveira
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  9. Helen Treichel
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  10. Débora de Oliveira
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Corresponding author

Correspondence to Helen Treichel.

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Richetti, A., Leite, S.G.F., Antunes, O.A.C. et al. Assessment of process variables on 2-ethylhexyl palmitate production using Novozym 435 as catalyst in a solvent-free system. Bioprocess Biosyst Eng 33, 331–337 (2010). https://doi.org/10.1007/s00449-009-0328-7

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  • DOI: https://doi.org/10.1007/s00449-009-0328-7

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