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Impact of different pH control agents on biopesticidal activity of Bacillus thuringiensis during the fermentation of starch industry wastewater

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Abstract

Different pH control agents (NaOH/H2SO4—SodSulp, NaOH/CH3COOH—SodAcet, NH4OH/CH3COOH—AmmoAcet and NH4OH/H2SO4—AmmoSulp) were used to investigate their effects on growth, enzyme production (alkaline protease and amylase), and entomotoxicity of Bacillus thuringiensis var. kurstaki HD-1 (Btk) against eastern spruce budworm larvae (Choristoneura fumiferana) using starch industry wastewater (SIW) as a raw material in a 15-l fermentor. AmmoSulp and SodSulp were found to be the best pH control agents for alkaline protease and amylase production, respectively; whereas, the fermented broth obtained by using SodAcet as pH control agents recorded the highest delta-endotoxin production of 1043.0 mg/l and entomotoxicity value 18.4 × 109 SBU/l. Entomotoxicity of re-suspended centrifuged pellet in one-tenth of original volume in case of SodAcet as pH control agents was 26.7 × 109 SBU/l and was the highest value compared to three other pH control agents.

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Acknowledgments

The authors are sincerely thankful to the Natural Sciences and Engineering Research Council of Canada (Grant A4984, Canada Research Chair) for financial support. The views and opinions expressed in this article are strictly those of authors. Sincere thanks to Dr. Satinder K. Brar and Dr. Simon Barnabé for reading and providing suggestions to prepare the manuscripts.

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Authors and Affiliations

  1. INRS-ETE, Université du Québec, 490, Rue de la Couronne, Quebec, QC, G1K 9A9, Canada

    Khanh Dang Vu, R. D. Tyagi & J. R. Valéro

  2. US EPA, P.O. Box-17-2141, Kansas, KS, 66117, USA

    R. Y. Surampalli

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  1. Khanh Dang Vu
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  2. R. D. Tyagi
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  3. J. R. Valéro
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  4. R. Y. Surampalli
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Correspondence to R. D. Tyagi.

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Vu, K.D., Tyagi, R.D., Valéro, J.R. et al. Impact of different pH control agents on biopesticidal activity of Bacillus thuringiensis during the fermentation of starch industry wastewater. Bioprocess Biosyst Eng 32, 511–519 (2009). https://doi.org/10.1007/s00449-008-0271-z

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