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Kinetic study of Bacillus thuringiensis var. israelensis in lab-scale batch process

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Abstract

Fermentations of Bacillus thuringiensis have long traditions for the production of δ-endotoxin as bioinsecticide in agriculture and forestry. A process analysis study of endoprotein production by batch fermentation is presented in this paper. General fermentation characteristics are given and the influence of dissolved oxygen to sporulation and toxicity is discussed. Kinetics of growth and product formation is examined and a model based on Monod with an inhibition constant is used to describe the process. The model should provide a useful framework for further process verification and optimisation in practice.

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Abbreviations

μ h−1 :

specific growth rate

μ max h−1 :

maximum specific growth rate

Ac g/l:

acetate concentration

Bti :

Bacillus thuringiensis var. israel ensis

CDW g/l:

cell dry weight

CPR mmol/sec:

carbon dioxide production rate

f t :

time factor

ITU :

international toxicity unit

K Ac g/l:

saturation constant for acetate

K IAc g/l:

inhibition constant for acetate

K O g/l:

saturation constant for oxygen

K Pr g/l:

saturation constant for protein

K S g/l:

saturation constant for glucose

O g/l:

dissolved oxygen concentration

OD :

optical density at 625 nm

OCR mmol/sec:

oxygen consumption rate

P g/l:

product concentration (spore count)

Pr g/l:

protein concentration

PHB :

poly hydroxy butyrate

r x g/l h:

biomass production rate

S g/l:

glucose concentration

t m h:

maturation time

vvm l /l h:

volume air per volume liquid of the reactor per minute

X g/l:

biomass concentration

Y P/S g/g:

gram of product produced per gram glucose consumed

Y P/X g/g:

gram of product per gram biomass

Y X/S g/g:

gram of biomass produced per gram glucose consumed

Y X/O g/g:

gram of biomass produced per gram oxygen consumed

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Authors and Affiliations

  1. Institute Biochemistry and Food Chemistry, Technical University Graz, Petersgasse 12, A-8010, Graz, Austria

    A. Kraemer-Schafhalter

  2. Institute of Biotechnology, Technical University Graz, Petersgasse 12, A-8010, Graz, Austria

    A. Moser

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  1. A. Kraemer-Schafhalter
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  2. A. Moser
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The authors would like to thank Bayer AG for carrying out the bioassays. This project research was supported by the Austrian FFWF under the Grant No P7695-Bio.

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Kraemer-Schafhalter, A., Moser, A. Kinetic study of Bacillus thuringiensis var. israelensis in lab-scale batch process. Bioprocess Engineering 14, 139–144 (1996). https://doi.org/10.1007/BF00369431

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  • DOI: https://doi.org/10.1007/BF00369431

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