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Preparation and characterization of chitosan membranes by using a combined freeze gelation and mild crosslinking method

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Abstract

When gelification is performed by freezing–thawing repeated cycles, the resultant gel-like polymer systems are called cryogels. This work aims to assess the effect of the addition of glutaraldehyde and 18 Crown Ether-6 on surface properties and protein loading of dried chitosan cryogel films. Residual water content of treated chitosan membranes ranged between 11.93 and 13.86%, while their water activities vary from 0.5 to 0.7 (measured from 4 to 60 °C). Based on thermal data, water evaporation peak and degradation temperatures of chitosan membranes shifted to a higher temperature for crosslinked samples. X-ray diffractograms provide high values of crystallinity for all the samples (70.67–92.86%), the highest value being for the glutaraldehyde-treated membrane. Candida rugosa lipase can be immobilized successfully on chitosan membranes. Lipase immobilized on glutaraldehyde-crosslinked chitosan yielded the highest efficiency in terms of total coupled protein and protein loading efficiency.

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Acknowledgments

The authors are grateful to Dr. Alfonso Devia C. (Laboratorio de Física del Plasma, Universidad Nacional-Manizales) for the AFM and XRD data and to Dr. Andres Rosales R. (Laboratorio de Magnetismo, Universidad Nacional-Manizales) for performing DSC and TGA analysis.

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  1. Departamento de Física y Química, Universidad Nacional de Colombia-Sede Manizales, Plantas Piloto de Biotecnología y Agroindustria, Campus La Nubia, Manizales, AA 127, Colombia

    Carlos E. Orrego

  2. Centro de Catálisis Heterogénea, Departamento de Química, Universidad Nacional de Colombia-Sede Bogotá, Ciudad Universitaria Tv 38 No. 40-01, Bogotá, AA 14490, Colombia

    Jesús S. Valencia

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Correspondence to Carlos E. Orrego.

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Orrego, C.E., Valencia, J.S. Preparation and characterization of chitosan membranes by using a combined freeze gelation and mild crosslinking method. Bioprocess Biosyst Eng 32, 197–206 (2009). https://doi.org/10.1007/s00449-008-0237-1

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