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Control of yeast fed-batch process through regulation of extracellular ethanol concentration

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Abstract

At high growth rates, the biomass yield of baker’s yeast (Saccharomyces cerevisiae) decreases due to the production of ethanol. For this reason, it is standard industrial practice to use a fed-batch process whereby the specific growth rate, μ, is fixed at a level below the point of ethanol production, i.e., μcrit. Optimally, growth should be maintained at μcrit, but in practice, this is difficult because μcrit is dependent upon strain and culture conditions. In this work, growth was maintained at a point just above μcrit by regulating ethanol concentration in the bioreactor. The models used for control design are shown, as are the experimental results obtained when this strategy was implemented. This technique should be applicable to all microorganisms that exhibit an “overflow” type metabolism.

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Acknowledgements

Funding by the Swiss National Science Foundation is gratefully acknowledged.

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Authors and Affiliations

  1. Laboratory of Chemical and Biochemical Engineering (LGCB), Swiss Federal Institute of Technology (EPFL), CH-1015, Lausanne, Switzerland

    Christopher Cannizzaro, Sergio Valentinotti & Urs von Stockar

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  1. Christopher Cannizzaro
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  2. Sergio Valentinotti
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  3. Urs von Stockar
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Correspondence to Urs von Stockar.

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Cannizzaro, C., Valentinotti, S. & von Stockar, U. Control of yeast fed-batch process through regulation of extracellular ethanol concentration. Bioprocess Biosyst Eng 26, 377–383 (2004). https://doi.org/10.1007/s00449-004-0384-y

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  • DOI: https://doi.org/10.1007/s00449-004-0384-y

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