Abstract
Industrial fermentations carried out in a 500-1 bioreactor were monitored on-line by a prototype of a split-flow modified thermal biosensor. Acetaldehyde and glycerol in the extracellular broth were monitored over the first 48 h of fed-batch fermentations. The aim was to determine the usefulness of these secondary metabolites for on-line monitoring and control. When fermentation of the 13–16 g/l batch sugar was monitored, using immobilised aldehyde dehydrogenase, the acetaldehyde reached a peak value of 0.3 g/l. With immobilised alcohol oxidase a much larger peak of 3.5 g/l ethanol was seen immediately after the acetaldehyde peak. When glycerokinase was used a delayed peak of 1 g/l glycerol was monitored. Of the three metabolites monitored, the ethanol proved the most valuable indicator of suitable timing for the start of the feeding phase and later for controlling and preventing overfeed using the on-line biosensor system.
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References
Christian, GD, Ruzicka J (1992) Exploiting stopped-flow injection analysis for quantitative chemical analysis. Anal Chim Acta 261:11–21
Danielsson, B (1991) Enzyme thermistor devices, In: Blum, LJ, Coulet, PR (eds) Biosensors principles and applications. Dekker New York, pp 83–105
Dantigny, P, Ninow, JL, Marc, I, Engasser, JM (1989) Representation of changes in the metabolic pattern of Baker's yeast from measurements of extracellular pyruvate, acetate, acetaldehyde and ethanol. Biotechnol Lett 7:515–520
Denis, CL, Ferguson, J, Young, ET (1982) mRNA levels for the fermentative alcohol dehydrogenase of Saccharomyces cerevisiae decrease upon growth on a nonfermentable carbon source. J of Biol Chem 258:1165–1171
Fiechter, A, Käppeli, O, Meussdorffer, F (1987) Batch and continuous culture. In: Rose, AH, Harrison, JS (eds) the Yeasts, vol 2. Academic Press, London, pp 99–129
Mosbach, K, Danielsson, B (1974) An enzyme thermistor. Biochim Biophys Acta 364:140–145
Oura, E (1977) Reaction products of yeast fermentations Process Biochem 4:19–21,35
Postma, E, Verduyn, C, Scheffers, A, Dijken van, JP (1989) Enzymic analysis of the Crabtree effect in glucose-limited chemostat cultures of Saccharomyces cerevisiae Appl Environ Microbiol 2:468–477
Rank, M, Gram, J, Danielsson, B (1992) Implementation of a thermal biosensor in a process environment: on-line monitoring of penicillin V in production scale fermentations. Biosensors, Bioelectron 7:5–8
Rank, M, Gram, J, Danielsson, B (1993) Industrial on-line monitoring of penicillin V, glucose and ethanol using a split-flow modified thermal biosensor. Anal Chim Acta 281:521–526
Ratledge, C (1991) Yeast physiology — micro-synopsis. Bioprocess Eng 6:195–203
Ratledge, C, Evans, CT (1987) Lipids and their metabolism. In: Rose, AH, Harrison, JS (eds), The yeasts, vol 3. Academic Press, London, pp 327
Scheper, T, Brandes, W, Grau, C, Hundeck, HG, Reinhardt, B, Ruther, F, Plötz, F, Schelp, C, Schügerl, K, Schneider, KH, Griffhorn, F, Rehr, B, Sahm, H (1991) Application of biosensor systems for bioprocess monitoring Anal Chim Acta 249:25–34
Stanley, GA, Pamment, NB (1993) Transport of intracellular accumulation of acetaldehyde in Sacharomyces cerevisiae. Biotechnol Bioeng 42:24–29
Weetall, HH (1976) Covalent coupling mehods for inorganic support materials Methods Enzymol 44:134–148
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Rank, M., Gram, J., Stern Nielsen, K. et al. On-line monitoring of ethanol, acetaldehyde and glycerol during industrial fermentations with Saccharomyces cerevisiae . Appl Microbiol Biotechnol 42, 813–817 (1995). https://doi.org/10.1007/BF00191174
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DOI: https://doi.org/10.1007/BF00191174