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Localization of myogenin, c-fos, c-jun, and muscle-specific gene mRNAs in regenerating rat skeletal muscle

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Abstract

It has been suggested that myogenin is an important factor for the differentiation of myoblasts and that its function in myogenesis is regulated by proto-on-cogenes in in vitro experiments. We have characterized the spatial and temporal expression patterns of myogenin, c-fos, c-jun, and muscle creatine kinase mRNAs during the skeletal muscle regeneration process using in situ hybridization histochemistry. Myogenin transcripts are first detected in the myonuclei/nuclei of satellite cells at 6 h after induction of regeneration. Myogenin mRNA is expressed in desmin-positive myoblasts, yet no muscle creatine kinase mRNA is detected in this cell type. Both the muscle creatine kinase and myogenin mRNAs are expressed in the newly formed myotubes, but not at earlier stages. Transcripts for c-fos and c-jun mRNAs are expressed first in the myonuclei/nuclei of satellite cells at 3 h post-trauma. c-jun mRNA is expressed in both myoblasts and myotubes, while c-fos mRNA was not detected in these cells. These results suggest that myogenin plays important roles in the regeneration of injured muscle and that c-jun and c-fos may have different roles in this process.

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Authors and Affiliations

  1. Department of Health Science, Osaka University of Health and Sport Sciences, 1558-1 Noda, Kumatori-cho, Sennan-gun, 590-04, Osaka, Japan

    Katsuya Kami

  2. Department of Anatomy and Neurobiology, Hyogo Medical College, 1-1 Mukogawa-cho, 633, Nishinomiya City, Hyogo, Japan

    Koichi Noguchi

  3. Department of Anatomy and Neurobiology, Wakayama Medical College, 27 Kyuban-cho, 640, Wakayama City, Wakayama, Japan

    Emiko Senba

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  1. Katsuya Kami
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  2. Koichi Noguchi
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  3. Emiko Senba
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Kami, K., Noguchi, K. & Senba, E. Localization of myogenin, c-fos, c-jun, and muscle-specific gene mRNAs in regenerating rat skeletal muscle. Cell Tissue Res 280, 11–19 (1995). https://doi.org/10.1007/BF00304506

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  • DOI: https://doi.org/10.1007/BF00304506

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