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β integrin-like protein-mediated adhesion and its disturbances during cell cultivation of the mussel Mytilus trossulus

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Abstract

In this study, we focus on the specific contribution of β integrin-like protein to adhesion-mediated events in molluscan larval cells in culture that could not have been investigated within the whole animal. An analysis of disturbances to cell–substratum adhesion, caused by the integrin receptor inhibiting Arg-Gly-Asp-Ser (RGDS)-peptide, the Ca2+/Mg2+-chelators and the stress influence of freezing–thawing, reveals that all these factors resulted in the partial destruction of the integrin–extracellular matrix (ECM) interaction in culture and, in particular, changes in the distribution and relative abundance of β integrin-positive cells. The experiments, carried out on selected substrates, found that β integrin-positive cells demonstrate different affinities for the substrates. This finding further supports the assumption that epithelial differentiation in cultivated cells of larval Mytilus may be mediated by β integrin-like proteins via binding to laminin; direct binding to other components of the ECM could not be demonstrated. The mussel β integrin-positive cells are not involved in myogenic or neuronal differentiation on any of the substrates but part of them has tubulin-positive cilia, forming some epithelia-like structures. Our data indicate that β integrin-positive cells are able to proliferate in vitro which suggests that they could participate in renewing the digestive epithelium in larvae. The findings provide evidence that the distribution pattern of β integrin-like protein depends on the cell type and the factors influencing the adhesion.

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Acknowledgments

This study was supported in part by the Russian Science Foundation (grant no. 14-14-00035). The work was performed at the “Far Eastern electron microscopy center” (A.V. Zhirmunsky Institute of Marine Biology, FEB RAS, Vladivostok, Russia). The authors express their thanks to Dr. A.V. Boroda and Dr. V.A. Dyachuk (A.V. Zhirmunsky Institute of Marine Biology, FEB RAS) for discussions and their help with experiments.

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  1. A. V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, Palchevsky Str. 17, 690041, Vladivostok, Russia

    Mariia A. Maiorova & Nelly A. Odintsova

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  1. Mariia A. Maiorova
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  2. Nelly A. Odintsova
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Correspondence to Nelly A. Odintsova.

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Maiorova, M.A., Odintsova, N.A. β integrin-like protein-mediated adhesion and its disturbances during cell cultivation of the mussel Mytilus trossulus . Cell Tissue Res 361, 581–592 (2015). https://doi.org/10.1007/s00441-015-2122-y

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