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The Lin28/let-7a/c-Myc pathway plays a role in non-muscle invasive bladder cancer

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Abstract

We investigate the role of the Lin28/let-7a/c-Myc pathway in non-muscle invasive bladder cancer (NMIBC). Using RT-PCR, western blot and immunohistochemistry techniques, the levels of pre-let-7a, let-7a, Lin28 and c-Myc RNA and/or proteins were determined in samples of normal bladder tissue and bladder cancer. Expression of pre-let-7a was found to be negatively correlated with the pathological grade of bladder cancer, while let-7a showed a positive correlation with bladder cancer pathological grade. Expression of Lin28 RNA and protein was not significantly different between normal bladder tissue and low-grade transitional cell carcinoma of bladder (TCC) but the expression levels in high-grade TCC were remarkably increased. Expression of c-Myc RNA and protein was significantly higher in bladder cancer samples in comparison to normal bladder tissue without correlation with cancer differentiation. Expression of all the above RNAs and proteins showed no significant difference in Ta and T1 stages. The Lin28/let-7a/c-Myc pathway plays an important role in NMIBC. In particular, expression levels of let-7a correlate with the degree of cancer differentiation but not cancer stage.

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Acknowledgments

This research was supported by a child-subject of the National High and New Technology Research and Development Program (863 Plans) (2007AA022006).

Competing interests

The authors have declared that no competing interest exists.

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Authors and Affiliations

  1. Department of Urology, the First Affiliated Hospital, Jinan University, Avenue West 601, Guangzhou, Guangdong, 510630, People’s Republic of China

    Yunfei Li, Caiyong Lai, Xinghua Du & Zexuan Su

  2. The Post-Graduate School of Southern Medical University, Guangzhou, Guangdong, 510515, People’s Republic of China

    Yunfei Li

  3. Guangdong Women and Children Hospital, Guangzhou, Guangdong, 511400, People’s Republic of China

    Haitao Liu

  4. Department of Pathology, Yuebei People’s Hospital, Shaoguan, Guangdong, 512026, People’s Republic of China

    Shuangquan Gao

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  1. Yunfei Li
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  4. Xinghua Du
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Correspondence to Zexuan Su.

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Yunfei Li and Haitao Liu made equal contributions to the paper.

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Li, Y., Liu, H., Lai, C. et al. The Lin28/let-7a/c-Myc pathway plays a role in non-muscle invasive bladder cancer. Cell Tissue Res 354, 533–541 (2013). https://doi.org/10.1007/s00441-013-1715-6

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  • DOI: https://doi.org/10.1007/s00441-013-1715-6

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