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Transplantation of bone-marrow-derived mesenchymal and epidermal stem cells contribute to wound healing with different regenerative features

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Abstract

A new concept for wound therapy is the initiation of the regeneration of epidermal and dermal layers with appendages for skin function recovery. Bone-marrow-derived mesenchymal and epidermal stem cells (BMSCs and SSCs) are hypothesized to be able to home toward or to be transplanted to wound sites for skin repair and regeneration, but this awaits confirmation by further experimental and clinical evidence. In this study, the influence of the transplantation of BMSCs and SSCs with porous gelatin-β-tricalcium phosphate sponge as scaffolds on wound re-epithelization, collagen synthesis, skin tensile strength recovery, and skin appendage regeneration has been investigated. The transplantation of BMSCs or SSCs significantly accelerates wound re-epithelization, stimulates dermal collagen synthesis, and exhibits the trend to enhance the tensile strength recovery of skin. Furthermore, regenerative features of BMSCs and SSCs have been identified in activating blood vessel and hair follicle formation, respectively. These results not only provide experimental evidence for the application of BMSCs and SSCs as promising therapeutics for clinical wound treatment, but also display their characteristics in activating distinct skin appendage regeneration, which might have novel applications in skin tissue engineering.

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Authors and Affiliations

  1. Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, 310058, Hangzhou City, People’s Republic of China

    Li-Hua Peng, Zu-Yao Mao, Xiao-Tian Qi, Xi Chen, Ni Li & Jian-Qing Gao

  2. Department of Biomedical Engineering, Weil Hall, Cornell University, Ithaca, N.Y., USA

    Zu-Yao Mao

  3. Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan

    Yasuhiko Tabata

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  1. Li-Hua Peng
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  2. Zu-Yao Mao
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  4. Xi Chen
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  5. Ni Li
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  6. Yasuhiko Tabata
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  7. Jian-Qing Gao
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Correspondence to Jian-Qing Gao.

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This research was supported by the National Natural Science Foundation of China (Grants No: 81102393, 30873173), the 48th China Postdoctoral Science Foundation (Grant No: 420000-X91004), the Fundamental Research Funds for the Central Universities, China, the the Basic Research Funds for the Zhejiang University and the Zhejiang Provincial Program for the Cultivation of High-Level Innovative Health Talents.

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Peng, LH., Mao, ZY., Qi, XT. et al. Transplantation of bone-marrow-derived mesenchymal and epidermal stem cells contribute to wound healing with different regenerative features. Cell Tissue Res 352, 573–583 (2013). https://doi.org/10.1007/s00441-013-1609-7

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  • DOI: https://doi.org/10.1007/s00441-013-1609-7

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