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Defining the morphological phenotype: 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) is a novel marker for in situ detection of canine but not rat olfactory ensheathing cells

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Abstract

Olfactory ensheathing cells (OECs) are the non-myelinating glial cells of the olfactory nerves and bulb. The fragmentary characterization of OECs in situ during normal development may be due to their small size requiring intricate ultrastructural analysis and to the fact that available markers for in situ detection are either expressed only by OEC subpopulations or lost during development. In the present study, we searched for markers with stable expression in OECs and investigated the spatiotemporal distribution of CNPase, an early oligodendrocyte/Schwann cell marker, in comparison with the prototype marker p75NTR. Anti-CNPase antibodies labeled canine but not rat OECs in situ, while Schwann cells and oligodendrocytes were positive in both species. CNPase immunoreactivity in the dog was confined to all OECs throughout the postnatal development and associated with the entire cell body, including its finest processes, while p75NTR was mainly detected in perineural cells and only in some neonatal OECs. Adult olfactory bulb slices displayed CNPase expression after 4 and 10 days, while p75NTR was detectable only after 10 days in vitro. Finally, treatment of purified adult canine OECs with fibroblast growth factor-2 significantly reduced CNPase expression at the protein and mRNA level. Taken together, we conclude that CNPase but not p75NTR is a stable marker suitable for in situ visualization of OECs that will facilitate their light-microscopic characterization and challenge our general view of OEC marker expression in situ. The fact that canine but not rat OECs expressed CNPase supports the idea that glia from large animals differs substantially from rodents.

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Acknowledgements

This study was supported by a grant to W.B. (BA 815/10-1) from the Deutsche Forschungsgemeinschaft (Research Unit 1103, Project 3). We thank P. Brünig for excellent technical assistance, K. Rohn for help with the statistical analysis, and A. Lehmbecker (Fraunhofer Institute for Toxicology and Experimental Medicine, Hannover, Germany) for providing adult rat tissue.

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  1. Department of Functional and Applied Anatomy, Hannover Medical School, Carl-Neuberg-Str.1, 30625, Hannover, Germany

    Mohamed Omar & Konstantin Wewetzer

  2. Department of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17, 30559, Hannover, Germany

    Patricia Bock, Robert Kreutzer, Susanne Ziege, Ilka Imbschweiler, Florian Hansmann, Claas-Tido Peck, Wolfgang Baumgärtner & Konstantin Wewetzer

  3. Center of Systems Neuroscience, Bünteweg 17, 30559, Hannover, Germany

    Florian Hansmann, Wolfgang Baumgärtner & Konstantin Wewetzer

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  1. Mohamed Omar
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Correspondence to Konstantin Wewetzer.

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Omar, M., Bock, P., Kreutzer, R. et al. Defining the morphological phenotype: 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) is a novel marker for in situ detection of canine but not rat olfactory ensheathing cells. Cell Tissue Res 344, 391–405 (2011). https://doi.org/10.1007/s00441-011-1168-8

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