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BMP2, BMP4, and their receptors are expressed in the differentiating muscle tissues of mouse embryonic tongue

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Abstract

To investigate the role of bone morphogenetic proteins (BMPs) in the differentiation process of skeletal muscle, we analyzed the in vivo expression of BMP2 and BMP4, of BMP receptors (BMPR) IA, IB, and II, and of activin receptors (ActR) IA, II, and IIB in mouse tongue muscle between embryonic day 11 (E11) and E17. The mRNA expression levels for BMP2 were 5-fold to 11-fold greater than those for BMP4 between E13 and E17 (P < 0.05-0.01). Expression of the BMP2, BMPRIB, ActRIA, ActRII, and ActRIIB proteins was first observed at E13. Expression of BMP2 and BMPRIB was detected in the whole area of the differentiating muscle tissues identified by immunostaining for fast myosin heavy chain (fMHC), but that of ActRIA, ActRII, and ActRIIB was detected only in the peripheral area of the differentiating muscle tissues. In the E15 tongue, all of the BMPs, BMPRs, and ActRs studied herein were expressed in the whole area of the differentiating muscle tissues identified by immunostaining for fMHC. These results suggest that BMPs play a role in the differentiation of tongue muscle tissues at E15 but have little or no effect at E13.

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Authors and Affiliations

  1. Department of Geriatric Dentistry, Tsurumi University School of Dental Medicine, Yokohama, Japan

    Takeo Suga & Mitsuhiko Morito

  2. Department of Orthodontics, Tsurumi University School of Dental Medicine, Yokohama, Japan

    Tadayoshi Fukui

  3. Waseda University Graduate School of Human Sciences, Tokorozawa, Japan

    Akiko Shinohara

  4. Department of Oral Biology, UIC College of Dentistry, Chicago, IL, USA

    Xianghong Luan & Thomas G. H. Diekwisch

  5. Department of Pharmacology, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama, 230-8501, Japan

    Akira Yamane

Authors
  1. Takeo Suga
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  2. Tadayoshi Fukui
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  3. Akiko Shinohara
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  4. Xianghong Luan
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  5. Thomas G. H. Diekwisch
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  6. Mitsuhiko Morito
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  7. Akira Yamane
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Corresponding author

Correspondence to Akira Yamane.

Additional information

This study was supported in part by grants-in-aid for funding scientific research (no. 16591871 to A.Y.) from the Bio-ventures and High-Technology Research Center of the Ministry of Education, Culture, Sports, Science, and Technology of Japan and by the Science Research Promotion Fund from the Promotion and Mutual Aid Corporation for Private Schools of Japan.

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Suga, T., Fukui, T., Shinohara, A. et al. BMP2, BMP4, and their receptors are expressed in the differentiating muscle tissues of mouse embryonic tongue. Cell Tissue Res 329, 103–117 (2007). https://doi.org/10.1007/s00441-007-0416-4

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  • DOI: https://doi.org/10.1007/s00441-007-0416-4

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