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Gene expression, cellular localization, and enzymatic activity of diacylglycerol kinase isozymes in rat ovary and placenta

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Abstract

Female reproductive organs show remarkable cyclic changes in morphology and function in response to a combination of hormones. Evidence has accumulated suggesting that phosphoinositide turnover and the consequent diacylglycerol (DG) protein kinase C (PKC) pathway are intimately involved in these mechanisms. The present study has been performed to investigate the gene expression, cellular localization, and enzymatic activity of the DG kinase (DGK) isozymes that control the DG-PKC pathway. Gene expression for DGKα, -ε, -ζ, and -ι was detected in the ovary and placenta. Intense expression signals for DGKζ and -α were observed in the theca cells and moderate signals in the interstitium and corpora lutea of the ovary. On the other hand, signals for DGKε were seen more intensely in granulosa cells. In the placenta, signals for DGKα and -ι were observed in the junctional zone, whereas those for DGKζ were detected in the labyrinthine zone. At higher magnification, the signals for DGKα were mainly discerned in giant cytotrophoblasts, and those for DGKι were found in small cytotrophoblasts of the junctional zone. DGKζ signals were observed in all cellular components of the labyrinthine zone, including mesenchyme, trabecular trophoblasts, and cytotrophoblasts. DGKε signals were detected in the junctional zone on day 13 and 15 of pregnancy and were diffusely distributed both in the labyrinthine and junctional zones at later stages. The present study reveals distinct patterns of mRNA localization for DGK isozymes in the rat ovary and placenta, suggesting that each isozyme plays a unique role in distinct cell types in these organs.

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Authors and Affiliations

  1. Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Iida-nishi 2-2-2, Yamagata, 990-9585, Japan

    Mayumi Toya, Yasukazu Hozumi, Tsukasa Ito, Morihiko Takeda & Kaoru Goto

  2. Department of Obstetrics and Gynecology, Yamagata University School of Medicine, Iida-nishi 2-2-2, Yamagata, 990-9585, Japan

    Mayumi Toya & Hirohisa Kurachi

  3. Department of Medicine, Tohoku University Graduate School of Medical Science, Seiryo-cho 2-1, Sendai, 980-8575, Japan

    Morihiko Takeda

  4. Department of Biochemistry, Sapporo Medical University School of Medicine, South-1, West-17, Sapporo, 060-8556, Japan

    Fumio Sakane & Hideo Kanoh

  5. Department of Perinatal Medicine and Maternal Care, National Center for Child Health and Development, Okura 2-10-1, Setagaya-ku, Tokyo, 157-8535, Japan

    Hidekazu Saito

  6. Yamagata Prefectural University of Health Sciences, Kamiyanagi 210, Yamagata, 990-2212, Japan

    Masahiko Hiroi

  7. Department of Cell Biology, Division of Histology, Tohoku University Graduate School of Medical Science, Seiryo-cho 2-1, Sendai, 980-8575, Japan

    Hisatake Kondo

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  1. Mayumi Toya
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  2. Yasukazu Hozumi
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  3. Tsukasa Ito
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  4. Morihiko Takeda
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  5. Fumio Sakane
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  6. Hideo Kanoh
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  7. Hidekazu Saito
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  8. Masahiko Hiroi
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  9. Hirohisa Kurachi
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  10. Hisatake Kondo
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  11. Kaoru Goto
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Correspondence to Kaoru Goto.

Additional information

This work was supported by Grants-in-Aids from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan, the Uehara Memorial Foundation, the ONO Medical Research Foundation, the Ciba-Geigy Foundation (Japan) for the Promotion of Science, the Kato Memorial Bioscience Foundation, and the Yamagata Health Support Foundation (to K.G.).

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Toya, M., Hozumi, Y., Ito, T. et al. Gene expression, cellular localization, and enzymatic activity of diacylglycerol kinase isozymes in rat ovary and placenta. Cell Tissue Res 320, 525–533 (2005). https://doi.org/10.1007/s00441-005-1089-5

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  • DOI: https://doi.org/10.1007/s00441-005-1089-5

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