Skip to main content

Advertisement

SpringerLink
Log in

Construction and characterization of bacterial artificial chromosome libraries from the silkworm, Bombyx mori

  • ORIGINAL PAPER
  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Abstract

Two bacterial artificial chromosome (BAC) libraries were constructed using nuclear DNA from posterior silkglands of the silkworm (Bombyx mori) strains p50 and C108. The libraries contain a total of 36,864 clones, or approximately 9 genome equivalents. The average insert sizes in the libraries were 134.5 kb and 120.8 kb, respectively. PCR-based screening was performed on the p50 library using probes for 34 sequence-tagged sites (STSs). Between 3 and 11 (6.1 hits on average) clones were isolated with each STS, in good agreement with the library size, 5.8 genome equivalents. The previously reported close linkage between the Bombyx homologs of the invected (Bm in) and engrailed (Bm en) genes was confirmed by construction of a BAC contig that contained both. Moreover, screening revealed novel information about the chromosomal organization of the sericin-1 and DH-PBAN genes, which were localized within a 22-kb interval and are divergently oriented. These results show that it is possible to construct contigs and analyze chromosome organization using these libraries.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. National Institute of Sericultural and Entomological Science (NISES), Owashi 1-2, Tsukuba, Ibaraki 305-8634, Japan Tel.: +81-298-386099; Fax: +81-298-386090, e-mail: yaskoch@nises.affrc.go.jp, , , , , , JP

    C. Wu & Y. Yasukochi

  2. Department of Molecular Biology, Keio University School of Medicine, Shinanomachi 35, Shinjuku-ku, Tokyo 160, Japan, , , , , , JP

    S. Asakawa & N. Shimizu

  3. National Institute of Agrobiological Resources (NIAR), Kan'non-dai 2-1-2, Tsukuba, Ibaraki 305-8602, Japan, , , , , , JP

    S. Kawasaki

Authors
  1. C. Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Asakawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. Shimizu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. Kawasaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Y. Yasukochi
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Received: 12 October 1998 / Accepted: 10 February 1999

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wu, C., Asakawa, S., Shimizu, N. et al. Construction and characterization of bacterial artificial chromosome libraries from the silkworm, Bombyx mori . Mol Gen Genet 261, 698–706 (1999). https://doi.org/10.1007/s004380050013

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s004380050013

Search

Navigation