Abstract
Cloned DNA libraries in bacterial artificial chromosome (BAC) are the most widely used form of large-insert DNA libraries. BAC libraries are typically represented by ordered clones derived from genomic DNA of a particular organism. In the case of large eukaryotic genomes, whole-genome libraries consist of a hundred thousand to a million clones, which make their handling and screening a daunting task. The labor and cost of working with whole-genome libraries can be greatly reduced by constructing a library derived from a smaller part of the genome. Here we describe construction of BAC libraries from mitotic chromosomes purified by flow cytometric sorting. Chromosome-specific BAC libraries facilitate positional gene cloning, physical mapping, and sequencing in complex plant genomes.
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Acknowledgment
This work was supported by grant LO1204 from the National Program of Sustainability I. We also thank Dr. Zbyněk Milec for creating figures and critical reading of the manuscript.
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Šafář, J., Šimková, H., Doležel, J. (2016). Construction of BAC Libraries from Flow-Sorted Chromosomes. In: Kianian, S., Kianian, P. (eds) Plant Cytogenetics. Methods in Molecular Biology, vol 1429. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3622-9_11
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DOI: https://doi.org/10.1007/978-1-4939-3622-9_11
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