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The barley plastome mutant CL2 affects expression of nuclear and chloroplast housekeeping genes in a cell-age dependent manner

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Abstract

The barley plastome mutant CL2 (cytoplasmic line 2) carries a point mutation in the infA gene, a homologue of the bacterial gene for the conserved translation initiator factor 1 (IF1). The function of infA in plastids is not known. The mutation in CL2 leads to a temporal chlorophyll deficiency in the primary leaf blade that is normalised in the basal and middle parts during further development. We have compared the expression of selected nuclear and plastid genes in different parts of primary leaves of CL2 and wild-type and found no indication for an adverse effect of the mutation on plastidial transcription. We observed an enhanced expression of RpoTp (encoding the phage-type nuclear-encoded plastid RNA polymerase) suggested to be caused by retrograde plastid signalling. Decreased amounts of plastid rRNA in basal and top sections are in agreement with the idea that the mutation in infA leads to a time- and position-dependent defect of plastid translation that causes a delay in plastid development. The normalisation of the phenotype in the middle section of CL2 leaves correlates with wild-type levels of chloroplast 16S rRNA and RbcL and increased expression of plastid housekeeping genes. The normalisation was not observed in cells at the tip of CL2 leaves suggesting different ways of regulating chloroplast development in cells at the tip of primary barley leaves as compared with other leaf sections.

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Acknowledgments

We are thankful to Juan J. Guiamet and Maureen Hanson for their generous gifts of antibodies. This work was supported by PICT 98: Proyecto de Investigación Científica y Técnica #04841, Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT), Argentina) to A.R.P. and by Deutsche Forschungsgemeinschaft (SFB 429) to T.B.

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Authors and Affiliations

  1. Instituto de Genética Ewald A. Favret, CICVyA, CNIA, INTA, CC 25, B1712WAA Castelar, Buenos Aires, Argentina

    Noemí Colombo & Alberto R. Prina

  2. Institute of Biology/Genetics, Humboldt University, Chausseestr.117, 10115, Berlin, Germany

    Noemí Colombo, Carola Emanuel & Thomas Börner

  3. Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria C1428EHA, Ciudad Autónoma de Buenos Aires, Argentina

    Verónica Lainez & Sara Maldonado

  4. Instituto de Recursos Biológicos, CIRN, CNIA, INTA, CC 25, B1712WAA Castelar, Buenos Aires, Argentina

    Sara Maldonado

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  1. Noemí Colombo
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  2. Carola Emanuel
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  3. Verónica Lainez
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  5. Alberto R. Prina
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  6. Thomas Börner
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Correspondence to Alberto R. Prina or Thomas Börner.

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Communicated by R. Herrmann.

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Colombo, N., Emanuel, C., Lainez, V. et al. The barley plastome mutant CL2 affects expression of nuclear and chloroplast housekeeping genes in a cell-age dependent manner. Mol Genet Genomics 279, 403–414 (2008). https://doi.org/10.1007/s00438-008-0321-x

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  • DOI: https://doi.org/10.1007/s00438-008-0321-x

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