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A transgressive segregation factor (RKN2) in Gossypium barbadense for nematode resistance clusters with gene rkn1 in G. hirsutum

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Abstract

Host plant resistance is an important strategy for managing root-knot nematode (Meloidogyne incognita) in cotton (Gossypium L.). Here we report evidence for enhanced resistance in interspecific crosses resulting from transgressive segregation of clustered gene loci. Recently, a major gene, rkn1, on chromosome 11 for resistance to M. incognita in cv. Acala NemX was identified using an intraspecific G. hirsutum cross with susceptible cv. Acala SJ-2. Using interspecific crosses of Acala NemX × susceptible G. barbadense cv. Pima S-7, F1, F2, F2:3, backcross, and testcross Acala NemX × F1 (Pima S-7 × SJ-2), parental entries and populations were inoculated in greenhouse tests with M. incognita. Genetic analyses based on nematode-induced root galling and nematode egg production on roots, and molecular marker analysis of the segregating interspecific populations revealed that gene rkn1 interacted with a gene (designated as RKN2) in susceptible Pima S-7 to produce a highly resistant phenotype. RKN2 did not confer resistance in Pima S-7, but when combined with rkn1 (genotype Aa or aa), high levels of resistance were produced in the F1 and segregating F2, F3, and BC1F1 populations. One SSR marker MUCS088 was identified tightly linked to RKN2 within 4.4 cM in a NemX × F1 (Pima S-7 × SJ-2) testcross population. Using mapped SSR markers and interspecific segregating populations, MUCS088 linked to the transgressive gene from the susceptible parent and was located in the vicinity of rkn1 on chromosome 11. Diverse genome analyses among A and D genome diploid and tetraploid cottons revealed that marker MUCS088 (165 and 167 bp) is derived from G. arboreum, A2 diploid genome. These results demonstrated that a highly susceptible parent contributed to nematode resistance via transgressive segregation. Derived highly resistant lines can be used as improved resistance sources in cotton breeding, and MUCS088 can be used to monitor RKN2 introgression in diverse populations. The close genomic location of the transgressive resistance determinants provides an important model system for studying transgressive segregation and epistasis in plants.

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Acknowledgments

This study was funded in part by a Cooperative Research Agreement from Cotton Incorporated and a grant from the University of California Discovery Grant (BioSTAR) Program. The authors thank Steven Oakley, James Starr, California Planting Cotton Seed Distributors, and USDA Cotton Germplasm Center for providing cotton seed, Mikeal Roose for helpful advice, and Kathie Carter, Sherry Ellberg, William Matthews, and Teresa Mullens for technical help. Names are necessary to report factually in available data, however, the USDA neither guarantees nor warrants the standard of products or service, and the use of the name by the USDA implies no approval of the product or service to the exclusion of others that may also be suitable.

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  1. Congli Wang

    Present address: Department of Nematology, University of California, Davis, CA, 95616, USA

Authors and Affiliations

  1. Department of Nematology, University of California, Riverside, CA, 92521-0415, USA

    Congli Wang & Philip A. Roberts

  2. USDA-ARS, Western Integrated Cropping Systems, Res. Unit, 17053 N. Shafter Ave., Shafter, CA, 93263, USA

    Mauricio Ulloa

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  1. Congli Wang
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Correspondence to Philip A. Roberts.

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Communicated by K. Shirasu.

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Wang, C., Ulloa, M. & Roberts, P.A. A transgressive segregation factor (RKN2) in Gossypium barbadense for nematode resistance clusters with gene rkn1 in G. hirsutum . Mol Genet Genomics 279, 41–52 (2008). https://doi.org/10.1007/s00438-007-0292-3

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